| 齐俊华,王展,石德玲,等.小有刺参硫酸软骨素和岩藻聚糖硫酸酯抗血小板聚集活性的比较[J].中国海洋药物,2019,38(1):42-48. |
| 小有刺参硫酸软骨素和岩藻聚糖硫酸酯抗血小板聚集活性的比较 |
| Comparison of anti-platelet aggregation activity of chondroitin sulfate and fucoidan from Holotoria floridona |
| 投稿时间：2018-05-20 修订日期：2018-07-06 |
| DOI： |
| 中文关键词: 硫酸软骨素 岩藻聚糖硫酸酯 低分子量样品 抗血小板聚集 凝血酶 |
| English Keywords:chondroitin sulfate fucoidan low-molecular-weight components anti-platelet aggregation thrombin |
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| 中文摘要: |
| 目的 从小有刺参中制备纯化硫酸软骨素和岩藻聚糖硫酸酯及其低分子量组分，并比较不同结构硫酸多糖的抗血小板聚集活性，揭示海参硫酸多糖抗血小板聚集活性的构效关系。方法 利用离子交换色谱和自由基降解技术，从小有刺参中制备硫酸软骨素和岩藻聚糖硫酸酯及各自的低分子量组分。采用体外凝血酶诱导血小板聚集模型，比较不同结构的硫酸软骨素和岩藻聚糖硫酸酯对血小板聚集率的影响。结果 4～60 μg/mL硫酸软骨素和低分子量硫酸软骨素具有显著的抑制血小板聚集的活性，在浓度为20 μg/mL时对血小板聚集的抑制率高达94.6%和95.3%，且分子量的降低对其活性无影响。比较不同来源的岩藻聚糖硫酸酯的活性发现，在4～60 μg/mL浓度范围内，海参与海带来源的岩藻聚糖硫酸酯都显著抑制血小板聚集，且活性没有显著差异，但经过自由基降解后，海参和海带来源的岩藻聚糖硫酸酯的抗血小板聚集活性都显著降低。结论 海参硫酸软骨素的抗血小板聚集活性显著高于岩藻聚糖硫酸酯，海参硫酸多糖可以应用于新型抗血小板聚集药物的研究开发。 |
| English Summary: |
| Objective The chondroitin sulfate，fucoidan and low-molecular-weight chondroitin sulfate and fucoidan from Holotoria floridona were prepared and their inhibitory effects on the platelet aggregation were compared to reveal the structure-activity relationship of anti-platelet aggregation activity of sulfated polysaccharides from Holotoria floridona. Methods The chondroitin sulfate and fucoidan and low-molecular-weight chondroitin sulfate and fucoidan were prepared by anion exchange chromatography and radical oxygen degradation from Holotoria floridona. The anti-platelet aggregation activity was assessed by thrombin-induced platelet aggregation model in vitro. Results 4～60 μg/mL chondroitin sulfate and low-molecular-weight chondroitin sulfate exhibited significant higher inhibitory effects on platelet aggregation, their inhibitory rate at 20 μg/mL on platelet aggregation reached 94.6% and 95.3%, respectively. And by comparison, the decrease in molecular weight of chondroitin sulfate did not affect the anti-platelet aggregation activity. The anti-platelet aggregation activity of fucoidans from Holotoria floridona and Laminaria japonica were compared and showed that at 4～60 μg/mL, the two fucoidans exhibited similar inhibitory activity on platelet aggregation induced by thrombin. However, the inhibitory activity of low-molecular-weight fucoidans obtained by radical oxygen degradation decreased significantly compared with fucoidans. Conclusion The anti-platelet aggregation activity of chondroitin sulfate and low-molecular-weight chondroitin sulfate were significantly higher than that of fucoidan from Holotoria floridona. Sulfate polysaccharides from sea cucumber had a potential as new anti-platelet drugs. |
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