姜芸,唐旭丽,王康熙,等.一株西沙来源软珊瑚共附生真菌Penicillium citrinum（15XS112ZM-18）次级代谢产物及其活性研究[J].中国海洋药物,2018,37(5):36-40.
一株西沙来源软珊瑚共附生真菌Penicillium citrinum（15XS112ZM-18）次级代谢产物及其活性研究
Secondary metabolites and their bioactivities of a soft coral-derived fungus Penicillium citrinum (15XS112ZM-18) collected from the Xisha Islands
投稿时间：2018-03-05  修订日期：2018-04-25
DOI：
中文关键词:  共附生真菌  P. citrinum  化学成分  生物活性
English Keywords:epiphytic fungi  P. citrinum  chemical constituents  bioactivity
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作者单位E-mail
姜芸 中国海洋大学 1843053460@qq.com 
唐旭丽 中国海洋大学  
王康熙 中国海洋大学  
李平林 中国海洋大学医药学院  
李国强* 中国海洋大学医药学院 liguoqiang@ouc.edu.cn 
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中文摘要:
      目的 研究1株中国西沙群岛永兴岛海域短指软珊瑚Sinularia cf. molesta共附生真菌Penicillium citrinum (15XS112ZM-18)的次级代谢产物及其生物活性。方法 利用TLC、硅胶柱层析、Sephadex LH-20凝胶柱层析、MPLC、半制备HPLC等方法，对菌株代谢物进行分离纯化；通过1H NMR及13C NMR等波谱学方法，结合相关文献数据，鉴定化合物结构；通过细胞毒活性模型评价化合物抗肿瘤活性。结果 从真菌P. citrinum (15XS112ZM-18)代谢产物中分离得到8个化合物，分别为：penicillenol A1 (1)，penicillenol A2 (2)，penicillenol B1 (3)，penicillenol B2 (4)，sorbicillin (5)，2"",3""-dihydrosorbicillin (6)，ergosterol (7)和25–Hydroxyergosta-4,6,8(14),22-tetraen-3-one (8)，其中化合物4在30 μmol L-1浓度下对HL-60细胞的抑制率达到100%，其IC50值为22.97 μmol L-1。
English Summary:
      Objective To investigate the secondary metabolites and their bioactivities of a Xisha soft coral Sinularia cf. molesta-derived fungus Penicillium citrinum (15XS112ZM-18). Methods The techniques of TLC, silica gel column, Sephadex LH-20 column chromatography, MPLC and HPLC were used to isolate and purify the compounds from the fermentation broth of P. citrinum (15XS112ZM-18). Their structures were elucidated by extensive NMR and MS spectroscopic data, and by comparison with the data of literature. The antitumor activities of the compounds were also evaluated by cytotoxicity experiment. Results Eight compounds were isolated from P. citrinum (15XS112ZM-18), and they were identified as penicillenol A1 (1), penicillenol A2 (2), penicillenol B1 (3), penicillenol B2 (4), sorbicillin (5), 2"",3""-dihydrosorbicillin (6), ergosterol (7)and 25–hydroxyergosta-4,6,8(14),22-tetraen-3-one (8). Among the eight componds, the inhibitory rate of 4 at the concentration of 30 μmol L-1 on HL-60 cells reached 100% with IC50 value 22.97 μmol L-1.
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