崔艳君,唐娜,牛建峰,等.岩藻黄素抗H2O2诱导WI-38细胞早衰的作用研究D*[J].中国海洋药物,2018,37(3):48-52.
岩藻黄素抗H2O2诱导WI-38细胞早衰的作用研究D*
Study on the anti-aging effect of fucoxanthin on H2O2-induced premature senescence in WI-38 human diploid fibroblasts
投稿时间：2018-01-17  修订日期：2018-03-28
DOI：
中文关键词:  岩藻黄素  WI-38细胞  H2O2  细胞早衰  抗衰老
English Keywords:Fucoxanthin  WI-38 cell  H2O2  cellular premature senescence  anti-aging
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作者单位E-mail
崔艳君 潍坊医学院 生物科学与技术学院 15589637567@163.com 
唐娜 潍坊医学院 生物科学与技术学院  
牛建峰 中国科学院海洋研究所  
朱敏敏 潍坊医学院 生物科学与技术学院  
成敏 潍坊医学院 临床医学院  
赵春玲 潍坊医学院 生物科学与技术学院  
綦慧敏 潍坊医学院 药学院  
刘顺梅* 潍坊医学院 生物科学与技术学院 liushm@wfmc.edu.cn 
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中文摘要:
      目的：探讨岩藻黄素是否具有抑制H2O2引起的WI-38细胞早衰的活性。方法：WI-38细胞在添加岩藻黄素的培养基中培养一定时间后经H2O2处理诱导发生早衰,用MTT法检测细胞活力,SA-β-半乳糖苷酶染色法检测细胞内衰老相关的β-半乳糖苷酶活性。 结果：300 μM H2O2处理WI-38细胞20 min可成功建立早衰细胞模型。与空白对照组相比,H2O2处理组细胞活力明显降低,SA-β-半乳糖苷酶阳性细胞数明显增多。5 μM和10 μM岩藻黄素组细胞活力明显高于H2O2处理组,SA-β-半乳糖苷酶阳性细胞数较H2O2组明显减少。 结论：岩藻黄素能够抑制由H2O2处理引起的细胞早衰,具有一定的抗衰老作用。
English Summary:
      Objective: The aim of this study is to investigate the anti-aging effect of fucoxanthin on H2O2-induced premature senescence in WI-38 human diploid fibroblasts. Methods: Cell viability tested by MTT and the morphological observation under microscope were used to screen the conditions for the establishment of H2O2-induced premature senescence WI-38 cell model. WI-38 cells were cultured in medium supplemented with different concentrations of fucoxanthin and then treated with H2O2 to induce premature senescence. Cell viability and senescence-associated β-galactosidase activity assessed by SA-β-galactosidase staining were tested to evaluate cellular senescence in different groups. Results: Premature senescence WI-38 cell model could be induced by treatment with 300μM H2O2 for 20 min. Compared with control group, the cell viability of H2O2-treated group was significantly decreased, and the number of SA-β-galactosidase positive cell was significantly increased. However, in the 5μM and 10μM FUCO groups, the cell viabilities were significantly higher than that of the H2O2-treated group, the numbers of SA-β-galactosidase positive cells were significantly decreased than that of the H2O2-treated group. Conclusion: FUCO could protect WI-38 cells from H2O2-induced premature senescence, it has anti-aging effect.
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