| 黄绵庆,陶桂兰,田树红,等.海莲叶提取物对胃癌细胞增殖的抑制作用及对小鼠急性毒性试验[J].中国海洋药物,2018,37(2):39-44. |
| 海莲叶提取物对胃癌细胞增殖的抑制作用及对小鼠急性毒性试验 |
| Inhibitory effect of bruguiera sexangula leaves extract on gastric cancer cells proliferation and its acute toxicity in mice |
| 投稿时间：2017-11-19 修订日期：2017-12-25 |
| DOI： |
| 中文关键词: 海莲 细胞增殖 急性毒性 细胞周期 |
| English Keywords:bruguiera sexangula cell proliferation acute toxicity cell cycle |
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| 中文摘要: |
| 目的 研究海莲叶提取物（BSLE）对人胃癌细胞增殖的抑制作用及其对小鼠的急性毒性。方法 先以CCK-8细胞增殖抑制试验测试BSLE对人胃癌细胞SGC-7901和MGC80-3的抑制作用。再以流式细胞术观察BSLE对人胃癌细胞周期的影响。然后建立人胃癌细胞SGC-7901和MGC80-3的裸小鼠皮下移植瘤模型，观察BSLE对胃癌细胞皮下移植瘤的抑制作用。最后将BSLE以9.6 g/kg剂量对小鼠单次给药，观察其急性毒性大小。结果 BSLE在细胞实验中剂量依赖性地抑制两种常见的胃腺癌细胞SGC-7901和MGC80-3的增殖，对这两种细胞的IC50分别为31.02 μg/mL和35.23 μg/mL。在裸小鼠抑瘤试验中，BSLE随着剂量增加对胃癌细胞皮下移植瘤生长的抑制作用逐渐增强，相对肿瘤增殖率（T/C）均低于40%。流式细胞术的结果显示BSLE能增加肿瘤细胞进入G0/G1期的细胞比率，提示它可能作用于G0/G1期。BSLE的急性毒性反应主要为腹泻和体重增长缓慢。结论 BSLE对胃癌细胞增殖有抑制作用，其作用机理可能与阻滞细胞G0/G1期有关。BSLE对小鼠的急性毒性反应较少且症状轻。 |
| English Summary: |
| Objective To investigate the inhibitory effect of bruguiera sexangula leaves extract(BSLE) on human gastric cancer cells proliferation and its acute toxicity in mice. Methods The inhibitory effect of BSLE on SGC-7901 cells and MGC80-3 cells was measured by CCK-8 assay. The effect of BSLE on cell cycle of these gastric cancer cells was observed by flow cytometry. Then, the subcutaneous transplanted tumor model of nude mice was established to observe the inhibitory effect of BSLE on subcutaneous tumor growth. Finally, the acute toxicity of BSLE was detected under 9.6 g/kg oral dose in mice. Results BSLE inhibited the proliferation of SGC-7901 cells and MGC80-3 cells in a dose-dependent manner, and the IC50 of BSLE on the two cells were 31.02 μg/mL and 35.23 μg/mL respectively. The inhibitory effect of BSLE on the growth of subcutaneous tumor of gastric cancer cells was enhanced with the increase of dosage, and the relative tumor proliferation rate (T/C) was less than 40%. The results from flow cytometry showed that BSLE could increase the cell rate of G0/G1 phase, suggesting that BSLE affected this phase possibly. BSLE"s acute toxicity was mainly diarrhea and slow weight gain. Conclusion BSLE has inhibitory effect on the proliferation of human gastric cancer cells, and its mechanism may be related to blocking G0/G1 phase. BSLE had less acute toxicity and induces mild symptoms in mice. |
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