王凯,王中红,李芳萍,等.孔石莼多糖及其硫酸酯化衍生物降血脂机制的体外研究[J].中国海洋药物,2018,37(2):81-87.
孔石莼多糖及其硫酸酯化衍生物降血脂机制的体外研究
The mechanism of Ulvapertusa and its derivative on decreasing Blood-lipid in vitro
投稿时间：2017-10-17  修订日期：2017-12-22
DOI：
中文关键词:  孔石莼多糖  硫酸酯化孔石莼多糖  人肝癌细胞  低密度脂蛋白受体
English Keywords:Ulva pertusa polysaccharide  sulfate derivative  Human liver cancer cells  Low density lipoprotein recepter
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作者单位E-mail
王凯 潍坊市第二人民医院 wfeyyjk@126.com 
王中红 潍坊医学院附属医院  
李芳萍 潍坊市第二人民医院  
高志强 潍坊市第二人民医院  
刘法荣 潍坊市第二人民医院  
綦慧敏* 潍坊医学院 wfqihuimin@126.com 
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中文摘要:
      目的 通过研究不同浓度孔石莼多糖(U)及硫酸酯化孔石莼多糖(HU)对人肝癌细胞HepG2中内源性低密度脂蛋白受体(LDLR)表达和LDLR mRNA转录的影响，探讨U和HU在受体及基因水平上的调脂作用机制。方法 利用Western-Blotting技术测定U和HU对HepG2细胞LDLR表达量的影响，采用荧光定量PCR技术分析不同浓度的U和HU对人肝癌细胞HepG2 LDLR mRNA表达的影响。结果HepG2细胞经不同浓度的U和HU作用后，U和HU用药组的LDLR表达量显著高于空白对照组(P<0.05)，证明从海藻孔石莼中提取的U和HU可显著提高人肝癌细胞HepG2中LDLR的表达水平。同时，HU药物组的LDLR表达量明显高于U药物组(P<0.05)。U和HU用药组其LDLR mRNA的含量与空白对照组相比均明显升高(P＜0.05)。其中U药物组(0.1 mg/mL)对HepG2细胞中LDLR mRNA的升高作用最明显，与空白对照组相比提高了约7.72倍。HU药物组(0.1 mg/mL)对HepG2细胞中LDLR mRNA的升高作用弱于U药物组(0.1 mg/mL)，与空白对照组相比提高了约3.89倍。结论 U和HU可能通过促进HepG2细胞中LDLR mRNA，增加肝细胞LDLR蛋白表达量，从而降低LDL浓度。
English Summary:
      Objective: To study the the possible mechanism of U and HU on decreasing blood-lipid,we had investigated the effect of U and HU on the expression of receptor (LDLR) and LDLR mRNA of the endogenous low density lipoprotein the human hepatomcellline HepG2 in vitro. Methods: We determinated the effect of U and HU on the expression of LDLR by western-Blotting technology and the expression of LDLR mRNA by fluorescence quantitative PCR technology in HepG2 cells. Results:We found that the expression of LDLR in U and HU groupwas significantly higher than blank control group (P < 0.05). The U and HU extracted from seaweed Ulva pertusahad obviously improved the expression of LDLR in HepG2.The expression of LDLR inthe HU group is higher than the Ugroup.By the fluorescence quantitative PCR technology，we found that the expression of LDLR mRNA in the group of U and HU is significantly higher than the blank control group (P < 0.05) . The expression of LDLR mRNA the U group (0.1 mg/mL)is increased about 7.72 times compared with the blank control group. The expression of LDLR mRNA of the HU group (0.1 mg/mL) isincreased about 3.89 times than the blank control group. But effect of U is stronger than HU on the expression of LDLR mRNA.
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