| 李媛媛,韩立华,鞠豪,等.海地瓜海参胶对胰岛素抵抗模型小鼠慢性炎症的改善作用[J].中国海洋药物,2018,37(2):32-38. |
| 海地瓜海参胶对胰岛素抵抗模型小鼠慢性炎症的改善作用 |
| The effect of Acaudina Molpadioide gelatin on ameliorating chronic inflammation in insulin resistance mice |
| 投稿时间：2017-08-28 修订日期：2017-11-28 |
| DOI： |
| 中文关键词: 慢性炎症 胰岛素抵抗 海地瓜海参胶 炎症因子 脂联素 |
| English Keywords:chronic inflammation insulin resistance Acaudina Molpadioide gelatin inflammatory cytokines adiponectin |
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| 中文摘要: |
| 目的 研究海地瓜海参胶对胰岛素抵抗模型小鼠慢性炎症的改善作用。方法 建立高脂高果糖饮食诱导胰岛素抵抗小鼠模型。雄性C57BL/6J小鼠随机分为正常对照组、模型对照组、海地瓜海参胶低剂量组（100 mg · (kg ·bw· d)-1）、海地瓜海参胶高剂量组（200 mg · (kg ·bw· d)-1）。饲喂9周后，检测小鼠空腹血糖、胰岛素、促炎因子游离脂肪酸、肿瘤坏死因子-α、白介素6及抗炎因子脂联素、白介素10水平；qRT-PCR 检测小鼠肝脏炎症因子mRNA表达水平。结果 海地瓜海参胶显著降低胰岛素抵抗模型小鼠空腹血糖、空腹胰岛素水平；显著降低血清促炎因子含量，升高抗炎因子含量；显著下调肝脏肿瘤坏死因子-α、白介素6 mRNA表达，上调脂联素、白介素10 mRNA表达。结论 海地瓜海参胶可显著改善胰岛素抵抗小鼠的慢性炎症。 |
| English Summary: |
| Abstract: Objective To investigate the effect of Acaudina Molpadioide gelatin on chronic low inflammation in insulin resistant mice. Methods The model of insulin resistance mice was established by feeding high fat and high fructose diet. Male C57BL / 6 mice were randomly divided into four groups: normal control group, model control group, Sea cucumber gelatin low dose group [100 mg·(kg·d)-1] and Sea cucumber gelatin high dose group [200 mg·(kg·d)-1]. All animals were fed with diet and water freely for 9 weeks．After 9 weeks treatment, the fasting serum glucose, fasting insulin levels, serum pro-inflammatory cytokines including free fatty acid, tumor necrosis factor-α, interleukin-6 and serum anti-inflammatory cytokines including adiponectin and interleukin 10 levels were tested. The mRNA expressions of tumor necrosis factor-α, interleukin-6, adiponectin and interleukin 10 in liver tissue were assayed with qRT-PCR. Results Sea cucumber gelatin significantly ameliorated the fasting blood glucose and insulin levels in insulin resistant mice; significantly decreased the secretions of pro-inflammatory cytokines and improved the secretions of anti-inflammatory cytokines; significantly down-regulated the mRNA expressions of tumor necrosis factor-α, interleukin-6and upregulated the mRNA expressions of adiponectin and interleukin-10 in liver. Conclusion Acaudina Molpadioide gelatin could significantly improve the chronic inflammation of insulin resistant mice. |
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