| 耿敏,苗贺,王春波,等.胶原蛋白肽经由HO-1途径保护H2O2诱导的MC3T3-E1细胞损伤△*[J].中国海洋药物,2018,37(1):25-30. |
| 胶原蛋白肽经由HO-1途径保护H2O2诱导的MC3T3-E1细胞损伤△* |
| Collagen peptide activates HO-1 signaling and protects MC3T3-E1 cells from H2O2 |
| 投稿时间：2017-07-05 修订日期：2017-09-11 |
| DOI： |
| 中文关键词: 胶原蛋白肽 过氧化氢 MC3T3-E1细胞 HO-1 |
| English Keywords:Collagen peptide Hydrogen peroxide MC3T3-E1 cells HO-1 |
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| 中文摘要: |
| 目的 骨质疏松症与氧化应激和活性氧（ROS）的产生密切相关，胶原蛋白肽具有潜在的抗氧化作用，其作用机理尚不清楚，因此，本研究在胶原蛋白肽对过氧化氢（H2O2）诱导的MC3T3-E1小鼠前成骨细胞氧化应激的保护作用的基础上，探讨其分子作用机制。方法 实验分为正常组，H2O2组，胶原蛋白肽低、中、高剂量组(10, 100, 500 g?L-1)。胶原蛋白肽各组加入相应浓度的药物预处理24 h后，与H2O2组一起加入400 μmol?L-1 H2O2孵育24 h，空白对照组正常培养。CCK 8和乳酸脱氢酶（LDH）的释放检测细胞毒性。抗氧化试剂盒检测细胞内活性氧（ROS）、谷胱甘肽过氧化物酶（GSH）和丙二醛(MDA)含量的变化，Western blot和RT-PCR分别检测细胞内HO-1蛋白及mRNA的表达水平。结果 胶原蛋白肽能够上调H2O2诱导的细胞存活率，以剂量依赖的方式显著降低H2O2诱导的氧化损伤。胶原蛋白肽能够及时清除细胞内的活性氧，显著上调HO-1的基因表达，提高GSH、MDA的活性，减轻脂质过氧化反应。结论 胶原蛋白肽通过激活HO-1基因表达水平，提高抗氧化活性，对H2O2诱导MC3T3-E1细胞的氧化损伤发挥保护作用。 |
| English Summary: |
| Osteoporosis development is closely associated with oxidativestress and reactive oxygen species (ROS). Collagen peptide has potential antioxidant effects and its pharmacological action in osteoblast is not clearly understood. The present study aimed to clarify the protective effects and mechanisms of collagen peptide on hydrogen peroxide (H2O2) induced oxidativestress in MC3T3-E1 osteoblastic cells. Methods The groups were divided into control, model and three different dose groups of collagen peptide (10,100,500 g?L-1). After adding the corresponding concentration of collagen peptide for 24 h, the groups were incubated with 400 μmol?L-1 H2O2 for 24 h, and the blank control group was normal culture. CCK8 and lactate dehydrogenase (LDH) were released to detect cytotoxicity. Oxygen species (ROS), glutathione peroxidase (GSH) and malondialdehyde (MDA) were measured by anti-oxidation kit. The expression of HO-1 protein was detected by Western blot. RT-PCR was used to detect the expression of HO-1 mRNA. Results Collagen peptide pretreatment reversescell viability inhibition and reduced H2O2-induced oxidative damage in a dose-dependent manner significantly. Collagen peptide can remove intracellular reactive oxygen species. Western blot analysis showed that collagen peptide promoted HO-1 gene expression. Collagen peptide improved GSH and MDA activity to reduce lipid oxidation reaction. Conclusion Collectively, collagen peptide activate HO-1 signaling and protect osteoblasts from hydrogen peroxide. Collagen peptide might have translational value for the treatment of oxidative stress associated osteoporosis. |
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