| 史姣霞,李敬,齐俊华,等.壳聚糖-岩藻聚糖硫酸酯纳米粒的制备及性质研究[J].中国海洋药物,2017,36(6):67-73. |
| 壳聚糖-岩藻聚糖硫酸酯纳米粒的制备及性质研究 |
| Preparation and property of chitosan-fucoidan nanoparticles |
| 投稿时间：2017-06-05 修订日期：2017-06-21 |
| DOI： |
| 中文关键词: 壳聚糖 岩藻聚糖硫酸酯 纳米粒 制备工艺 |
| English Keywords:Chitosan Fucoidan Nanoparticles Preparation technology |
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| 中文摘要: |
| 目的：本文以低分子量岩藻聚糖硫酸酯为交联剂,研究了聚电解质凝聚法制备壳聚糖-岩藻聚糖硫酸酯纳米微粒(Chitosan-fucoidan nanoparticles, CS-Fuc NPs)的制备工艺,并对纳米粒的胃肠道和贮藏稳定性进行研究。方法：采用聚电解质凝聚法制备壳聚糖-岩藻聚糖硫酸酯纳米微粒,采用体外模拟胃液和模拟肠液消化体系,检测纳米粒的胃肠稳定性,常温贮藏实验测定纳米粒的短期贮藏稳定性。结果：壳聚糖-岩藻聚糖硫酸酯纳米微粒的最优制备条件是：壳聚糖(1mg/ml)与岩藻聚糖硫酸酯(1mg/ml)体积比为1.1/1,pH 4.5,温度30℃。所得纳米粒粒径为227.8 nm,Zeta电位为38.4 mV,PDI为0.231,岩藻聚糖硫酸酯复合率(Fuc%)为94.92%。所制备的纳米颗粒在10周内没有显著变化,在模拟胃环境中稳定性良好,在模拟肠道环境中解聚性良好。结论： 壳聚糖-岩藻聚糖硫酸酯纳米粒制备工艺简单,性能良好,有望成为新型的口服药物运送载体。 |
| English Summary: |
| Objective: To optimize the polyelectrolyte complexation technology of chitosan-fucoidan nanoparticles by using the low weight molecular fucoidan as a crosslinking agent and study its gastrointestinal stability and storage stability. Methods: Polyelectrolyte complexation was used to prepare the nanoparticles. Gastrointestinal stability of the nanoparticles was measured by in vitro simulated digestion and storage stability was determined by storage at 25℃. Results: When the chitosan/fucoidan ratio was 1.1/1, temperature was 30℃ and pH was 4.5, the optimum preparation conditions was achieved. The optimum nanoparticles had an average diameter of 227.8nm, a Zeta potential of 38.4 mV, a PDI of 0.231 and a fucoidan loading rate( Fuc% ) of 94.92%. The chitosan-fucoidan nanoparticles demonstrated no significant changes within 10 week, and it was stable in simulated gastric environment and had a good depolymerization simulated intestinal environment. Conclusion: The chitosan-fucoidan nanoparticles have a simple preparation process and good nature, it can be used as a new carriier for oral drugs. |
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