| 李瑞英,马岩,刘颖,等.海兔素对高铁暴露大鼠脂质代谢和肠黏膜损伤的保护作用[J].中国海洋药物,2017,36(4):54-60. |
| 海兔素对高铁暴露大鼠脂质代谢和肠黏膜损伤的保护作用 |
| Protective effects of Aplysin on lipid metabolism and intestinal mucosal damage of rats exposed by excessive iron |
| 投稿时间：2017-02-24 修订日期：2017-04-14 |
| DOI： |
| 中文关键词: 海兔素 高铁 脂质代谢 肠道屏障 肠道菌群 |
| English Keywords:Aplysin excessive iron lipid metabolism intestinal barrier intestinal flora |
| Fund Project: |
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| 中文摘要: |
| 目的 建立高铁暴露大鼠模型,探讨海兔素(Aplysin)对高铁暴露大鼠脂质代谢和肠黏膜损伤的保护作用。方法 48只Wistar雄性大鼠随机分为4组,A组为正常对照组(基础饲料,铁含量50mg/kg)、B组为高铁模型组(高铁饲料,铁含量1000mg/kg)、C组为海兔素对照组(基础饲料 海兔素灌胃150mg/kg)、D组为海兔素 高铁组(高铁饲料 海兔素灌胃150mg/kg),实验周期为12w。HE染色观察小肠组织形态结构；测定血清铁、肝组织铁、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、二胺氧化酶(DAO)、D-乳酸(D-LA)、肠脂肪酸结合蛋白(FABP2)等含量；荧光实时定量PCR(RT-PCR)检测大鼠粪便大肠杆菌、粪肠球菌、双歧杆菌、乳酸杆菌、脆弱拟杆菌、柔嫩梭菌含量。结果 与A组比较,B组大鼠血清铁和肝组织铁含量分别升高46.9%和44.5 %(P＜0.05),与B组比较,D组大鼠血清铁和肝组织铁含量均显著降低(P＜0.05)。HE染色结果显示,A组和C组肠粘膜组织正常,结构完整,无损伤性改变。B组大鼠肠绒毛和腺体均明显受损,大量粘膜上皮细胞脱落,肠绒毛出血、水肿,长期海兔素干预后得到显著改善。与A组比较,B组大鼠血清TG、LDL-C和血浆D-LA、FABP2含量均显著升高(p<0.05)。D组大鼠血清TG、LDL-C和血浆D-LA、FABP2含量较B组均显著降低(p<0.05)。另外,B组大鼠血浆DAO和血清TC含量较A组均有上升,D组大鼠血浆DAO和血清TC含量较B组均有下降,但均无显著性(p>0.05)。RT-PCR结果显示,与A组比较,B组大鼠粪便大肠杆菌数量显著升高,双歧杆菌、乳酸杆菌和柔嫩梭菌数量均显著降低(P＜0.05)。与B组比较,D组大鼠粪便大肠杆菌数量显著降低,双歧杆菌和柔嫩梭菌数量均显著升高(P＜0.05),B组大鼠粪便粪肠球菌和脆弱拟杆菌数量较A组均有升高,但经统计学处理,均无显著性(p>0.05)。与B组比较,D组大鼠粪便粪肠球菌和脆弱拟杆菌数量均有降低趋势,乳酸杆菌数量有升高趋势,但均无显著性(p>0.05)。而C组大鼠所测各指标与A组均无明显差异(p>0.05)。结论 海兔素对高铁暴露所致大鼠脂质代谢紊乱和小肠粘膜损伤具有良好的调节和保护作用。 |
| English Summary: |
| Abstract: Objective To establish a model of rats exposed by excessive iron and to investigate the effect of Aplysin on lipid metabolism and intestinal mucosal damage of rats exposed by excessive iron. Methods Forty-eight male wistar rats which were randomly divided into the following four groups: control group (A), received basic diet; iron group (B), received diet containing Fe2 1000mg/kg; Aplysin group (C), administered daily Aplysin dose of 150 mg/(kg?bw?d); Aplysin iron group (D), received diet containing Fe2 1000mg/kg and administered daily Aplysin dose of 150 mg/(kg?bw?d). The experiment lasted for twelve weeks. The histopathology of small intestine was observed after hematoxylin-eosin (HE) staining; Serum and liver iron concentration, total cholesterol (TC)，triglyceride (TG), low density lipoprotein cholesterol (LDL-C), diamine oxidese (DAO), D-lactate (D-LA), fatty acid binding protein (FABP2)were detected. The real-time PCR (RT-PCR) method was utilized to determine the levels of Escherichia coil, Enterococcus, Bifidobacterium, Lactobacillus, Bacteroides fragils and Clostridium tender. Results Compared with A group, serum iron and liver iron concentrations of B group were increased significantly 46.9% and44.5 % respectively（P＜0.05）. Serum iron and liver iron concentration of D group were decreased significantly compared with B group（P＜0.05）. The results of HE staining indicated that intestinal mucosal tissue of rats in A group and C group are complete. After rats were exposed by iron in a long-term time, intestinal villus and glands were damaged significantly, a large number of mucosal epithelial cell falls off, mucosal epithelium and lamina propria separation, intestinal villus hemorrhage, edema. However, the pathological injuries were improved with the intervention of Aplysin. Compared with A group, all of serum TG、LDL-C and plasma D-LA、 FABP2 levels in B group were elevated significantly （P＜0.05）. The levels of serum TG、LDL-C and plasma D-LA、FABP2 in D group were all decreased significantly compared with B group（P＜0.05）. In addition, the levels of plasma DAO and serum TC of B group were all elevated compared with A group, the levels of plasma DAO and serum TC of D group were all reduced compared with B group, but no significant differences（p>0.05）. As RT-PCR indicated, compared with A group, the level of Escherichia coil of B group was increased significantly （P＜0.05）, the levels of Bifidobacterium、Lactobacillus and Clostridium tender of B group were all decreased significantly（P＜0.05）. Compared with B group, the level of Escherichia coil of D group was reduced significantly（P＜0.05）, the levels of Bifidobacterium、Lactobacillus and Clostridium tender of D group were all elevated significantly（P＜0.05）. The levels of Enterococcus and Bacteroides fragils of B group were increased compared with A group, the levels of Enterococcus and Bacteroides fragils of D group were reduced compared with B group, the level of Lactobacillus of D group was increased compared with B group, but all of these changes no significant differences（p>0.05）. And there was no significant difference between A group and C group in all these indicators （p>0.05）. Conclusion Aplysin exhibited regulative and protective effect on lipid metabolism disorder and intestinal mucosal damage induced by excessive iron exposure in rats. |
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