崔华,李晓丹,李明岐,等.海绵共生真菌Penicillium janthinellum LZDX-32-1抗乙型肝炎病毒次生代谢产物研究[J].中国海洋药物,2017,36(3):41-46.
海绵共生真菌Penicillium janthinellum LZDX-32-1抗乙型肝炎病毒次生代谢产物研究
Anti-HBV secondary metabolites from sponge-associated fungus Penicillium janthinellum LZDX-32-1CUI Hua1, LI Xiao-dan2, LI Ming-qi4 , LU Feng-min3,WANG Yuan-hua1
投稿时间：2017-01-12  修订日期：2017-03-14
DOI：
中文关键词:  Xestospongia testudinaria  Penicillium janthinellum  次生代谢产物  HepG2.2.15细胞  抗HBV活性
English Keywords:Xestospongia testudinaria  Penicillium Janthinellum  secondary metabolites  HepG2.2.15cells  anti-HBV activity
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作者单位E-mail
崔华 潍坊医学院药学院 cuihua_0606@163.com 
李晓丹 北京大学 天然药物及仿生药物国家重点实验室  
李明岐 平度市第三人民医院  
鲁凤民 北京大学 基础医学院
北京大学 基础医学院 		 
王元花 潍坊医学院药学院  
刘东 北京大学 天然药物及仿生药物国家重点实验室  
康建功* 潍坊医学院药学院 kjg63@163.com 
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中文摘要:
      目的 探究Xest,ospongia testudinaria海绵共生真菌Penicillium janthinellum LZDX-32-1的次生代谢产物及其抗乙型肝炎病毒(HBV)活性。方法 对菌株进行发酵,运用现代色谱学方法(硅胶柱色谱、凝胶柱色谱、C-18反相柱色谱,以及半制备HPLC)对发酵产物进行分离,运用现代波谱学技术(核磁共振、质谱、紫外等),结合文献数据对比鉴定了化合物的结构；采用HepG2.2.15细胞模型的HBV DNA 水平用实时定量 PCR测定评价化合物的抗HBV活性。结果 分离鉴定了11个化合物,分别为：penialidin C (1)、penialidin A(2)、6-(2,4-dihydroxy-6-methylphenyl)-4-hydroxy-2-pyrone(3)、trans-3,4-dihydro-3,4,8-trihydroxynaphthalen-1(2H)-one(4)、epi-?isoshinanolone (5)、 pyrenocine B(6)、FK17-P2b1(7)、cordyol C(8)、diorcinol(9)、p-acetamidobenzoic acid(10)、2-(2-oxoindolin-3-yl)-acetamide(11)。 活性评价结果报道化合物5显示有抗HBV活性。结论 Xestospongia testudinaria海绵共生真菌 Penicillium janthinellum LZDX-32-1能够代谢产生具有抗HBV活性的次生代谢产物。
English Summary:
      Objective To investigate the bioactive secondary metabolites of Xestospongia testudinaria associated fungus Penicillium janthinellum LZDX-32-1. Methods After larger scaled fermentation, the secondary metabolites were isolated from the crude extracts of culture broth based on column chromatography over silica gel, Sephadex-LH 20, C-18 ODS and semi-prepared HPLC. Their structures were elucidated through spectroscopic analysis of UV, ESI-MS, 1D and 2D NMR, as well as by comparison with the data of literatures. The anti-HBV activities were assayed using HBV mRNA level testing with reverse-transcript PCR in Hep G2.2.15 cells, Results Eleven compounds were isolated and identified as penialidin C(1), penialidin A(2),6-(2,4-dihydroxy-6-methylphenyl)-4-hydroxy-2-pyrone(3),trans-3,4-dihydro-3,4,8-trihydroxynaphthalen-1(2H)-one(4), epi-?isoshinanolone(5), pyrenocine B(6), FK17-P2b1(7), cordyol C(8), diorcinol(9), p-acetamidobenzoic acid(10), 2-(2-oxoindolin-3-yl)-acetamide(11). In vitro bioassay showed that compound 5 exhibited anti-HBV activity. Conclusion The fermentation of strain LZDX-32-1 could produce secondary metabolites with anti-HBV antivity.
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