| 史筱莉,徐永健,牟金婷,等.大海马和刁海龙氨基酸与脂肪酸的组成分析与评价[J].中国海洋药物,2017,36(2):75-83. |
| 大海马和刁海龙氨基酸与脂肪酸的组成分析与评价 |
| Analysis of amino acids and fatty acids of Hippocampus kuda and Solenognathus hardwickii |
| 投稿时间：2016-09-21 修订日期：2016-11-13 |
| DOI： |
| 中文关键词: 大海马 刁海龙 氨基酸 脂肪酸 分析与评估 |
| English Keywords:Hippocampus kuda Solenognathus hardwickii Amino acid Fatty acid Nutrition evaluation |
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| 中文摘要: |
| 目的 研究野生大海马、养殖大海马以及刁海龙三者间氨基酸与脂肪酸的组成和含量差异，旨在为野生海马的高效利用及其适宜替代生物的探寻提供依据。方法 氨基酸自动分析仪测定氨基酸含量，气相色谱-质谱联用仪（GC-MS）测定脂肪酸组成与含量。结果 野生与养殖大海马和刁海龙均含有测定的17种氨基酸，含量范围在43.207～74.827 g/100g DW之间，其中养殖大海马的含量最高，刁海龙次之，野生大海马最低；大海马皮膜的氨基酸含量要高于骨粉，而刁海龙则相反；三者限制性氨基酸中均包含亮氨酸，必需氨基酸指数均较高。刁海龙的不饱和脂肪酸含量高于大海马，尤其是EPA和DHA，占总脂肪酸含量的25.41%和32.74%，远高于后者的2.55%和3.87%；其余脂肪酸相近；养殖大海马与野生大海马间的差异不大。结论 野生大海马的皮膜与骨粉中氨基酸和脂肪酸组成与含量相近，可合理开发；养殖大海马在营养评价方面略优于刁海龙，可优先选择作为野生大海马的替代，刁海龙氨基酸与脂肪酸的营养品质也高于野生大海马，尤其是EPA和DHA含量显著高于野生大海马，可合理选择作为替代。 |
| English Summary: |
| Objective To provide proofs for higher-value utilization of wild seahorse resource and exploration of its substitute in traditional Chinese medicine, and analyze the amino acids (AA) and fatty acids (FA) from their cortex and bone of one type of seadragon (Solenognathus hardwickii) and two types of Hippocampus kuda (the cultured and the wild), respectively. Methods AA was determined by automatic amino acid analyzer, and FA was determined by Gas Chromatography-Mass Spectrometer (GC-MS). Results Three groups all contained 17 kinds of AA, total content was 43.207~74.827 g/100 g of dry matter, and the cultured seahorse had the highest content, the second was the seadragon, then the wild seahorse was the lowest within the 3 groups. AA content in seahorse’s cortex was higher than that in its bone, but opposite in the seadragon. EAAI in all groups was high, and Ile was one of the restrictive amino acids among them. UFA in the seadragon was higher than that in seahorse, especially EPA and DHA, whose contents in the seadragon were 25.41% and 32.74%, respectively, of its dry matter, and only 2.55% and 3.87% in the seahorse. Other fatty acids were similar between seahorse and seadragon. There were no distinct differences between the cultured and the wild seahorse. Conclusion AA and FA contents were similar between the cortex and the bone of the wild seahorse, which could provide for reasonable exploitation and utilization. In nutrients of AA and FA, the cultured seahorse was slightly better than the wild, could be preferred as an alternative in TCM, the seadragon was also better than the seahorse, especially in EPA and DHA, and was an alternative too. |
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