| 李德宝,张雪晴,张秀丽,等.一株花柳珊瑚来源真菌 Curvularia lunata 产 cytochalasin B 发酵优化及其化学表观遗传修饰研究D[J].中国海洋药物,2016,35(5):14-20. |
| 一株花柳珊瑚来源真菌 Curvularia lunata 产 cytochalasin B 发酵优化及其化学表观遗传修饰研究D |
| Fermentation optimization for the gorgonian coral-derived fungus Curvularia lunata to produce cytochalasin B and the study of adding chemical epigenetic modification in fermentation |
| 投稿时间：2015-09-07 修订日期：2015-09-08 |
| DOI： |
| 中文关键词: 柳珊瑚来源真菌 优化 cytochalasin B 丁酸钠 |
| English Keywords:gorgonian-derived fungus optimize cytochalasin B sodium butyrate |
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| 中文摘要: |
| 目的 通过对中国南海花柳珊瑚来源真菌 Curvularia lunata (RA16-1) 进行发酵优化以提高该菌的 cytochalasin B 产量,并加入组蛋白去乙酰化酶抑制剂丁酸钠以考察对该菌次级代谢产物的影响。方法 运用单因素试验设计,对菌株产cytochalasin B的碳源、氮源、初始pH 值、盐浓度等发酵条件进行优化；运用柱色谱层析技术对加入丁酸钠的真菌Curvularia lunata (RA16-1) 发酵产物进行分离,以及核磁与质谱技术对分离的化合物进行结构鉴定。结果 单因素试验表明最优发酵条件为：碳源为蔗糖 (30 g.L-1)、 氮源为蛋白胨 (30 g.L-1)、发酵时间为96 h、初始pH为5.0、盐浓度为 2%。此外,从加入丁酸钠的发酵产物中分离得到了4个化合物,其中两个化合物(化合物2和3)为首次从该物种中获得。结论 在优化条件下,cytochalasin B的产量可达到2.3g.L-1。组蛋白去乙酰化酶抑制剂丁酸钠的加入后使得该菌发酵产物的结构类型发生较大变化。 |
| English Summary: |
| Objective To increase the yield of cytochalasin B by optimizing the fermentation conditions for the gorgonian coral-derived fungus Curvularia lunata (RA16-1) and to detect the influence of secondary metabolites by adding the histone deacetylase inhibitor (HDACI)-sodium butyrate. Methods The single-factor experiment was designed to investigate the optimum fermentation conditions including carbon and nitrogen sources, pH, salinity and time. The organic extract of the fungus was separation and purification by column chromatography methods. The structures of the compounds were elucidated on the basis of NMR and MS experiments. Results The optimum fermentation conditions ：sucrose (30 g.L-1), peptone (30 g.L-1), pH 5.0, 96 h of fermentation, 2% of salinity；Additionally, four compounds were obtained from fermentation broth added sodium butyrate, two of which were isolated from this fungus for the first time. Conclusions The yield of cytochalasin B is improved to 2.3 g.L-1under optimal fermentation conditions. The type of the compounds isolated from Curvularia lunata (RA16-1) change very much by adding the histone deacetylase inhibitor (HDACI). |
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