丁琅,单鑫迪,郝杰杰,等.拟小叶喇叭藻和喇叭藻多糖对α-葡萄糖苷酶活性的影响[J].中国海洋药物,2016,35(3):81-86.
拟小叶喇叭藻和喇叭藻多糖对α-葡萄糖苷酶活性的影响
The α-Glucosidase inhibitory effects of polysaccharides from Turbinaria conoides and Turbinaria ornata
投稿时间：2015-07-16  修订日期：2015-08-30
DOI：
中文关键词:  拟小叶喇叭藻  喇叭藻  多糖  α-葡萄糖苷酶  抑制活性
English Keywords:Turbinaria conoides  Turbinaria ornata  polysaccharide  α-glucosidase  inhibitory activity
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作者单位E-mail
丁琅 中国海洋大学医院学院 dingl1206@163.com 
单鑫迪 中国海洋大学医院学院  
郝杰杰 中国海洋大学医院学院  
吕友晶 中国海洋大学医院学院  
凡飞 中国海洋大学医院学院  
赵小亮 中国海洋大学医院学院  
蔡超 中国海洋大学医院学院  
于广利* 中国海洋大学医院学院 glyu@ouc.edu.cn 
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中文摘要:
      摘 要：目的 研究喇叭藻多糖对α-葡萄糖苷酶抑制活性。方法 采用热水提取从拟小叶喇叭藻（Turbinaria conoides）和喇叭藻（Turbinaria ornata）中获得水溶性多糖TCP和TOP，并采用硫酸-苯酚法测定其总糖含量；用改良的咔唑法测定其糖醛酸含量；用Folin-酚法（Lowry法）测定其蛋白含量；用高效液相色谱法（HPLC）测定其单糖组成；用离子色谱法（IC）测定其硫酸根含量；用高效凝胶渗透色谱与多角度激光散射仪联用法（HPGPC-MALLS）测定其平均分子量；用PNPG显色法测定TCP和TOP对α-葡萄糖苷酶抑制活性。结果TCP和TOP具有良好的α-葡萄糖苷酶抑制活性，IC50值分别为204.1和113.2 μg?mL?1，效果优于阿卡波糖（220.3 μg?mL?1）。初步动力学研究表明其均为混合型抑制方式。结论 热水提取自拟小叶喇叭藻和喇叭藻的多糖具有良好的α-葡萄糖苷酶抑制活性。 关键词：拟小叶喇叭藻；喇叭藻；多糖；α-葡萄糖苷酶；抑制活性
English Summary:
      Abstract: Objective To study the α-glucosidase inhibitory activities of the polysaccharides from Turbinaria conoides and Turbinaria ornata. Methods Two polysaccharides, namely TCP and TOP, were obtained with hot-water extraction. The contents of total sugar, uronic acid, protein, monosaccharide and sulfate were determined by sulfuric acid-phenol method, modified carbazole method, Lowry assay, high performance liquid chromatography (HPLC) and ion chromatography (IC), respectively. Meanwhile, the average molecular weights (Mw) and α-glucosidase inhibitory activities of TCP and TOP were analyzed by HPGPC-MALLS and PNPG method. Results TCP and TOP showed potent inhibitory effects on α-glucosidase with the IC50 values of 204.1 and 113.2 μg?mL?1 respectively, which were better than those of acarbose (220.3 μg?mL?1). Preliminary kinetic study exhibited that the ?-glucosidase inhibitions were both in mixed-type manner. Conclusion Two polysaccharides extracted with hot water from Turbinaria conoides and Turbinaria ornata possessed good α-glucosidase inhibitory activities. Key words: Turbinaria conoides; Turbinaria ornata; polysaccharide; α-glucosidase; inhibitory activity
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