| 董奇,薛佳兴,卢忠华,等.静息细胞转化北沙参中香豆素类组分的研究[J].中国海洋药物,2016,35(2):41-50. |
| 静息细胞转化北沙参中香豆素类组分的研究 |
| Study on coumarin constituents of Glehnia littoralis by resting cells transformation |
| 投稿时间：2015-06-01 修订日期：2015-07-29 |
| DOI： |
| 中文关键词: 静息细胞 生物转化 香豆素 |
| English Keywords:resting cell biotransformation coumarin |
| Fund Project: |
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| 摘要点击次数: 4955 |
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| 中文摘要: |
| 目的 优化极地真菌S-3-66-E1转化北沙参中香豆素类组分的条件。方法 采用静息细胞转化法,考察了生物转化条件(菌体浓度、底物浓度、温度、pH、摇床转速、金属离子)对转化体系的影响,并通过正交实验优化工艺参数,利用HPLC检测总香豆素类组分的转化情况。结果 确定摇瓶转化的最优条件：培养温度15℃,摇床转速240r/min,细胞浓度60g.L-1,底物浓度0.1 g.L-1,pH值5.9,转化时间24h,在此条件下香豆素转化量趋近完全。结论 采用静息细胞法转化北沙参中香豆素类组分的工艺稳定可行。 |
| English Summary: |
| Objective To optimize the transformation conditions of coumarins components from Glehnia littoralis by polar fungus S-3-66-E1. Methods The effects of biological transformation conditions (cell concentration, substrate concentration, temperature, pH, rotation speed, metal ion) were optimized by orthogonal design, and detected by high performance liquid chromatography (HPLC). Results The optimal conditions for the transformation were determined as follows: culture temperature 15℃; rotation speed 240r/min cell concentration 60g.L-1; substrate concentration 0.1g.L-1; pH5.9; conversion time 24h.which the amount of coumarin transformed completely. Conclusion The transformation condition of the resting cell of coumarins components from Glehnia littoralis by Polar fungus S-3-66-E1 is stable and feasible. |
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