| 宋龙,吕律,王远红,等.罗氏海盘车(Asterias rollestoni)中两种生物碱的分离纯化及其抗肿瘤活性研究[J].中国海洋药物,2016,35(1):50-54. |
| 罗氏海盘车(Asterias rollestoni)中两种生物碱的分离纯化及其抗肿瘤活性研究 |
| The preparation and purification of two alkaloids derived from Asterias rollestoni and studies on their antitumor activities |
| 投稿时间：2015-05-21 修订日期：2015-05-26 |
| DOI： |
| 中文关键词: 罗氏海盘车 生物碱 抗肿瘤活性 非核糖体肽 |
| English Keywords:Asterias rollestoni alkaloid antitumor activity NRPSs |
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| 中文摘要: |
| 目的 从罗氏海盘车（Asterias rollestoni）中分离鉴定生物碱类天然产物，并进行体外抗肿瘤活性评价。方法 利用MCI GEL CHP20凝胶吸附树脂柱层析、硅胶薄层层析、硅胶柱层析、凝胶 Sephadex LH-20 柱层析和高效液相色谱等手段对化合物进行了分离纯化；运用NMR、MS等波谱方法并结合文献鉴定化合物的结构；采用SRB、MTT法评价化合物的抗肿瘤活性。结果 从罗氏海盘车的乙醇提取物中分离鉴定了1个环二肽生物碱类化合物 Fellutanine A （1）和1个吲哚生物碱N-（2-（1H-吲哚-3-基）乙基）-2-苯乙胺（2）。化合物1对MGC803细胞的抑制率为53.99%，具有一定的抗肿瘤活性。结论 两个生物碱类化合物均为首次从罗氏海盘车中分离获得，其中化合物1的发现说明罗氏海盘车中存在非核糖体肽合成酶，进一步研究其合成路线、从中分离非核糖体多肽合成酶可以为挖掘罗氏海盘车药用价值提供重要参考。 |
| English Summary: |
| Objective To isolate and identify alkaloids from Asterias rollestoni and evaluate their antitumor activities. Methods Isolation and purification were performed by MCI GEL CHP20 gel adsorption resin, TLC, silica gel column chromatography, Sephadex LH-20 column chromatography, and HPLC. Structural characterization was established by spectroscopic analysis (NMR, MS) and comparison with literature data. Antitumor activities were evaluated by SRB and MTT methods. Results Two alkaloids were isolated and identified as Fellutanine A (1) and N-(2-(1H-indol-3-yl)ethyl)-2-phenylethanamine (2). Compound 1 exhibited potent cytotoxic activity against MGC803 with the inhibition rate of 53.99%. Conclusion It’s the first report for both alkaloids isolated from Asterias rollestoni. The discovery of compound 1 proves the existence of nonribosomal peptide synthetases (NRPSs) in Asterias rollestoni. Studies on the synthetic route and separating NRPSs from Asterias rollestoni will be further researched to provide reference for gleaning medicinal value of Asterias rollestoni. |
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