范平龙,操红缨,陈家艺,等.海带海参降糖口服液对2型糖尿病大鼠肝组织中IRS-2及PTP-1BmRNA表达的影响[J].中国海洋药物,2016,35(1):60-64.
海带海参降糖口服液对2型糖尿病大鼠肝组织中IRS-2及PTP-1BmRNA表达的影响
The Influence of the Kelp Sea Cucumber Hypoglycemic Oral Liquid on the Expression of IRS-2 and PTP-1BmRNA in Type 2 Diabetic Rats
投稿时间：2015-05-18  修订日期：2015-11-12
DOI：
中文关键词:  海带海参降糖口服液  2型糖尿病  胰岛素受体底物-2  蛋白酪氨酸激酶激酶-1B
English Keywords:type 2 diabetic  the Hypoglycemic oral liquid about Stichopus japonicus and Laminaria japonica  IRS-2mRNA  PTP-1BmRNA
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作者单位E-mail
范平龙 广州中医药大学  
操红缨 广州中医药大学  
陈家艺 湖北民族学院附属民大医院 1031118320@qq.com 
赖焕玲 广州中医药大学  
黄思明 广州中医药大学  
梁志健 广州中医药大学  
练庆旺 广州中医药大学  
黄萍* 广州中医药大学 hping331@126.com 
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中文摘要:
      目的 观察海带海参降糖口服液对2型糖尿病大鼠的影响,检测肝组织中IRS-2及PTP-1BmRNA表达水平,从胰岛素转导的初始环节探讨其作用机制。方法 采用高糖高脂饲料结合链脲佐菌素制备2型糖尿病大鼠模型,观察海带海参降糖口服液对2型糖尿病大鼠空腹血糖值、血清中胰岛素含量的影响、计算胰岛素敏感性及胰岛素抵抗指数,RT-PCR检测肝组织中IRS-2及PTP-1BmRNA表达水平。结果 与模型组相比,海带海参降糖口服液能显著降低动物空腹血糖值、胰岛素抵抗指数以及肝组织中PTP-1BmRNA表达水平,增加血清中胰岛素含量、胰岛素敏感性及肝组织中IRS-2mRNA表达水平。结论 海带海参降糖口服液具有显著的改善作用,可能是通过影响胰岛素转导初始环节,上调肝组织中IRS-2mRNA表达水平以及下调PTP-1BmRNA表达水平得以发挥。
English Summary:
      Object To explore the mechanism of the Hypoglycemic oral liquid about Stichopus japonicus and Laminaria japonica treated type 2 diabetic mellitus (T2DM )rats,observation the expression of IRS-2 and PTP-1BmRNA in liver tissue of T2DM rats.Method Preparation T2DM rat model by high fat feed combined with streptozocin (STZ) inducing ,and then study the effects of the Hypoglycemic oral liquid about Stichopus japonicus and Laminaria japonica by observed the fasting blood glucose and the content of insulin in serum ,calculation the insulin sensitivity and insulin resistance, detected the expression of IRS-2 and PTP-1BmRNA in liver tissues by RT-PCR.Result Compared with the model group, the Hypoglycemic oral liquid about Stichopus japonicus and Laminaria japonica can significantly decrease the fasting blood glucose ,insulin resistance and the expression of PTP-1BmRNA , and increased the content of insulin in serum,insulin sensitivity and the expression of IRS-2mRNA in live tissue.Conclusion The Hypoglycemic oral liquid about Stichopus japonicus and Laminaria japonica has significant moderating effect on T2DM rats, maybe through up regulation of the expression of IRS-2mRNAand decrease the expression level of PTP-1BmRNA in lever .
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