文章摘要
盐碱地植物内生真菌SXH-69胞外多糖的分离、化学组成和结构特征
Isolation, chemical components and structural characterizations of extracellular polysaccharides produced by the saline-alkali plant endophytic fungus SXH-69
投稿时间:2015-01-12  修订日期:2015-02-05
DOI:
中文关键词: 盐碱地植物内生真菌  胞外多糖  分离  化学组成  结构特征
英文关键词: Saline-alkali plant endophytic fungus  extracellular polysaccharide  isolation  chemical component  structural characterization
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
郭涛 中国海洋大学医药学院 海洋药物教育部重点实验室 934110765@qq.com 
李洁 中国海洋大学  
郭甜甜 中国海洋大学  
孙新华 中国海洋大学  
李菁 中国海洋大学  
杨宝勤 中国海洋大学  
顾谦群 中国海洋大学  
毛文君 中国海洋大学医药学院 海洋药物教育部重点实验室 wenjunm@ouc.edu.cn 
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中文摘要:
      目的 对分离自滨州盐碱地植物内生真菌SXH-69的胞外多糖进行研究。 方法 利用乙醇沉淀法? Q Sepharose Fast Flow离子交换柱色谱和Sephacryl S-100凝胶柱色谱对菌株发酵液中的胞外多糖进行分离纯化;通过高效凝胶渗透色谱(HPGPC)? PMP柱前衍生高效液相色谱(HPLC)? 红外光谱(IR)及气质联用色谱(GC-MS)等方法,研究胞外多糖的化学组成和结构特征。 结果 从发酵液中分离纯化获得2个胞外多糖sx1-1和sx2-1,它们的分子量分别为13.5 kDa和18.3 kDa;这2个多糖主要由甘露糖组成,还含少量葡萄糖和半乳糖;sx1-1和sx2-1中均含有末端Man,1,4)-Man,1,6)-Man和1,2,4)-Man,而sx1-1还含有末端Glc, 1,2)-Gal,1,6)-Glc,1,3,6)-Glc;sx2-1中还含有1,2)-Glc,1,2,6)-Gal。 结论 首次从滨州盐碱地植物内生真菌SXH-69中分离得到胞外多糖sx1-1和sx2-1,它们有着不同的化学特征,是结构新颖的杂多糖。
英文摘要:
      Abstract: Objective To investigate the extracellular polysaccharides from the saline-alkali plant endophytic fungus SXH-69. Methods The extracellular polysaccharides were isolated from the culture medium of the saline-alkali plant endophytic fungus using ethanol precipitation, Q-Sepharose Fast Flow anion-exchange and Sephacryl S-100 gel-permeation chromatography. The chemical compositions and structural characteristics of the extracellular polysaccharides were studied by high gel permeation chromatography (HPGPC), pre-column high performance liquid (HPLC) derivation, Fourier-transform infrared (IR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Results Two polysaccharides, sx1-1 and sx2-1, were obtained from the culture medium of the saline-alkali plant endophytic fungus, and their molecular weights were about 13.5 and 18.3 kDa, respectively. The two polysaccharides were mainly composed of mannose. Minor amounts of glucose and galactose were also found. Methylation analysis showed that the extracellular polysaccharides sx1-1 and sx2-1 were mainly composed of terminal Man, 1,4)-Man, 1,6)-Man and 1,2,4)-Man, with some amounts of terminal Glc, 1,2)-Gal, 1,6)-Glc and 1,3,6)-Glc for sx1-1, and sx2-1 with 1,2)-Glc and 1,2,6)-Gal. Conclusion The two extracellular polysaccharides from the plant endophytic fungus SXH-69 was firstly isolated and investigated. The two extracellular polysaccharides had different chemical characteristics, and were heteropolysaccharides with novel structures.
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