| 刘全永,崔保伟 胡江春 薛德林 王书锦.海洋细菌Bacillus coagulans spp.heishijiaosis 发酵液抗真菌活性及其机制研究[J].中国海洋药物,2015,34(3):65-70. |
| 海洋细菌Bacillus coagulans spp.heishijiaosis 发酵液抗真菌活性及其机制研究 |
| Antifungal activity and mechanism of the bioactive substance derived from marine bacteria Bacillus coagulans spp. Heishijiaosis fermentation broth |
| 投稿时间：2014-12-10 修订日期：2015-01-29 |
| DOI： |
| 中文关键词: 海洋细菌 凝结芽胞杆菌 生物活性物质 抗真菌活性 |
| English Keywords:marine bacteria Bacillus coagulans anti-fungal bioactive substances |
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| 中文摘要: |
| 目的：对凝结芽胞杆菌黑石礁新亚种LU-B02产生的抗白色念珠菌活性物质的作用靶位及抑菌谱进行了研究。方法：采用山梨醇保护形态变异法结合扫描电镜超微结构观察研究了活性物质的作用靶位，最小抑菌浓度(MIC)法比较了该菌发酵液对不同真菌的抑菌活性。结果：该活性物质抑制白色念珠菌细胞壁中的主要组分——葡聚糖的合成从而造成其细胞壁残缺。该活性物质具有抗真菌专一性，对革兰氏阳性代表菌金黄色葡萄球菌和革兰氏阴性代表菌大肠杆菌等细菌均无抑制效果，而对人体病原真菌抑菌效果较为明显，尤其是对白色念珠菌的抑制效果较好。MIC测定表明白色念珠菌对LU-B02活性物质高度敏感，相当于斯皮仁诺8~16 μg，抑菌效价每毫升相当于3500 U制霉菌素或洁尔阴活性的4倍。发酵液在酸性条件下加入0.2 %冰片时，室温下存放6个月仍可保持良好的抗白色念珠菌活性。结论：凝结芽孢杆菌黑石礁新亚种产生的活性物质在控制以白色念珠菌为主的真菌感染方面具有良好应用前景。 |
| English Summary: |
| Aim: Study on the antimicrobial targets and spectrum of the bioactive substance from Bacillus coagulans spp. Heishijiaosis fermentation broth. Methods: The antimicrobial targets and activity were determined by sorbitol protection morphological variation combined with scanning electron microscope and the minimum inhibitory concentration (MIC), respectively. Results: The bioactive substance exhibited strong antifungal activity against fungi, especially Canidia albicans, and the target maybe owing to the inhibition dextran synthesis resulting in incomplete cell well. However, the bioactive substance had no effect on Gram-positive bacteria (Staphylococcus aureaus) and Gram-negative bacteria (Escherichia coli). The MIC value is equivalent to 8-16 μg Sporanox. The antifungal effect is 3500 U nystatin or 4 times of Jieryin (an antifungal Chinese medicine), respectively. The bioactive substance added 0.2 % borneol showed good activity against C. albicans after six month storage. Conclusion: The bioactive substance from B. coagulans fermentation broth posses good prospects in controlling C. albicans infections. |
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