文章摘要
海洋源放线菌Streptomyces sp. 223中二酮哌嗪类成分的研究
Study of piperazinedione derivatives from the marine-derived Streptomyces sp. 223
投稿时间:2014-11-04  修订日期:2015-01-06
DOI:
中文关键词: 海洋放线菌  次级代谢产物  二酮哌嗪类化合物  结构鉴定  细胞毒活性
英文关键词: Marine actinomycetes  secondary metabolites  piperazinedione  structure elucidation  cytotoxic activity
基金项目:
作者单位E-mail
曲承蕾 烟台大学 生命科学学院 ydqcl2007@163.com 
杨雪 山东国际生物科技园发展有限公司  
张淑敏 滨州医学院 药学院  
谢则平 滨州医学院 药学院  
金海珠 烟台大学 生命科学学院 jhz3008@sina.com 
摘要点击次数: 2304
全文下载次数: 1053
中文摘要:
      目的 对采集自烟台逛荡河入海口沉积物的一株放线菌Streptomyces sp. 223进行次级代谢产物及活性研究。方法 采用硅胶柱层析、Sephadex LH-20凝胶层析、薄层层析以及HPLC等方法对放线菌Streptomyces sp. 223发酵产物进行分离纯化,通过波谱数据分析及文献比较对化合物进行结构鉴定,并用MTT法初步评价其细胞毒活性。结果 从该菌的发酵产物中分离到了7个二酮哌嗪类化合物,分别鉴定为(3S)-(1,4)-二甲基-3-异丙基-(2,5)哌嗪二酮(1),环-(脯-苯丙)二肽(2),环(脯-酪)二肽(3),环(4-OH-脯-亮)二肽(4),环(苯丙-甘)二肽(5),环(4-OH-脯-苯丙)二肽(6)和环-(脯-缬)二肽(7)。结论 化合物1为首次从海洋微生物中分离得到的新颖天然产物,且具有显著的细胞毒活性,对Hela细胞的半抑制浓度IC50=18.7 μmol.L-1。
英文摘要:
      Objective To investigate the bioactive secondary metabolites from the marine-derived actinomycete Streptomyces sp. 223 isolated from a estuary sediment of Guangdang River, Yantai. Methods The fermentation products were separated via silica gel column chromatography, Sephadex LH-20, thin layer chromatography and HPLC. The structures of the purified compounds were elucidated on the basis of spectral analysis and comparison with the data in literatures. The cytotoxicities were determined by MTT method. Results Totally 7 compounds were separated and elucidated as (3S)-1,4-dimethyl-3-(1-methylethyl)- 2,5-piperazinedione (1), cyclo-(Pro-Phe) (2), cyclo-(Pro-Tyr) (3), cyclo-(4-hydroxyl-Pro-Leu) (4), cyclo-(Phe-Gly) (5), cyclo-(4-hydroxyl-Pro-Phe) (6), and cyclo-(Pro-Val) (7). Conclusion compound 1 was firstly isolated from marine actinomyces as a new natural product and showed cytotoxicities against Hela cell line with IC50 =18.7 μmol.L-1.
查看全文   查看/发表评论  下载PDF阅读器
关闭