孙世伟,付娟,周会楠,等.红树林来源黑曲霉Aspergillus niger ZHN2-20次级代谢产物的研究D[J].中国海洋药物,2014,33(6):1-7.
红树林来源黑曲霉Aspergillus niger ZHN2-20次级代谢产物的研究D
Studies on secondary metabolites from mangrove-derived fungus Aspergillus niger ZHN2-20
投稿时间：2014-04-29  修订日期：2014-07-01
DOI：
中文关键词:  红树林  黑曲霉  次级代谢产物  甾类化合物  细胞毒活性
English Keywords:mangrove  Aspergillus niger  secondary metabolites  steroids  cytotoxicity
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作者单位E-mail
孙世伟 教育部海洋药物重点实验室 sunshiwei_1986@163.com 
付娟 教育部海洋药物重点实验室  
周会楠 教育部海洋药物重点实验室  
朱天骄 教育部海洋药物重点实验室  
李德海 教育部海洋药物重点实验室  
毛文君 教育部海洋药物重点实验室  
顾谦群* 教育部海洋药物重点实验室 guqianq@ouc.edu.cn 
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中文摘要:
      目的 研究红树林植物榄李Lumnitzera racemosa根泥来源黑曲霉Aspergillus niger ZHN2-20的抗肿瘤活性次级代谢产物。方法 利用硅胶柱层析、凝胶Sephadex LH-20柱层析、HPLC等常规化学手段对次级代谢产物进行了分离纯化；通过理化性质结合波谱学数据分析及文献对照,鉴定化合物的结构；分别以SRB和MTT法评价了化合物的细胞毒活性。结果 从黑曲霉Aspergillus niger ZHN2-20的次级代谢产物中,分离鉴定了5个甾类单体化合物,分别为：helvolic acid (1),methyl helvolic acid (2),(22E, 24R)-ergosta-7, 9 (11), 22-triene-3β, 5α, 6β-triol (3),cerevisterol (4),(22E, 24R)-ergosta-7, 9 (11), 22-triene-3β, 5α-diol (5)。化合物3、5对HL-60和K-562肿瘤细胞株均表现出一定的细胞增殖抑制作用,抑制率分别为78.10%,60.50%和62.36%,49.43%。 结论 化合物1-3、5为首次在黑曲霉Aspergillus niger中分离得到,其中化合物2为新结构化合物。首次测定了化合物1-5对Hela、A-549、MGC-803、HL-60和K-562五种肿瘤细胞株的细胞毒活性,其中化合物3、5对两株人白血病细胞显示出一定的抑制作用。
English Summary:
      Objective To study the active secondary metabolites of Aspergillus niger ZHN2-20, a fungus which was isolated from the mangrove sediment around the Lumnitzera racemosa. Methods The isolation and purification of the compounds were performed by silica gel, Sephadex LH-20 and HPLC methods, their structures were determined by comparison their physicochemical characters and spectral data with literatures, and their cytotoxicities were evaluated by SRB and MTT methods respectively. Results Five steroidal compounds (1-5) were isolated from the Aspergillus niger ZHN2-20 and identified as helvolic acid (1), methyl helvolic acid (2), (22E, 24R)-ergosta-7, 9 (11), 22-triene-3β, 5α, 6β-triol (3), cerevisterol (4), (22E, 24R)-ergosta-7, 9 (11), 22-triene-3β, 5α-diol (5). Compounds 3 and 5 showed inhibitory activities against HL-60 and K-562 cells, with IR% values of 78.10%, 60.50% and 62.36%, 49.43%, respectively. Conclusion Compounds 1, 3 and 5 were isolated from this strain for the first time, and 2 was a new compound; Compounds 1-5 were evaluated their cytotoxicity against Hela, A-549, MGC-803, HL-60, and K-562 cell lines for the first time, and compounds 3 and 5 exhibited more potential inhibitory against two human leukemia cell lines.
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