文章摘要
太平洋牡蛎多糖的提取、分离和结构分析
Extraction, separation and structural characterization of polysaccharides from Crassostrea gigas
投稿时间:2014-01-19  修订日期:2014-02-20
DOI:
中文关键词: 牡蛎  多糖  提取  分离  结构分析
英文关键词: Crassostrea gigas  polysaccharide  extraction  separation  structural characterization
基金项目:国家海洋局公益性行业专项;国家“十二五”科技支撑计划
作者单位E-mail
高蒙蒙 中国海洋大学医药学院 kuding_gao@126.com 
赵峡 中国海洋大学医药学院 zhaoxia@ouc.edu.cn 
王清池 中国海洋大学医药学院  
栾晓红 中国海洋大学医药学院  
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中文摘要:
      目的 从太平洋牡蛎(Crassostrea gigas)肉中提取和分离纯化多糖,并对其基本理化性质和结构组成进行分析。方法 将牡蛎鲜肉制成丙酮粉,依次采用室温水,60 ℃热水和稀碱提取牡蛎粗多糖,对其总糖含量、蛋白含量和单糖组成进行分析。采用DEAE Sepharose FF阴离子交换和Sephacryl S-400凝胶柱层析对粗多糖进行分离纯化,并对获得的纯化组分采用红外光谱 (IR)、甲基化、气质联用 (GC-MS)、电喷雾质谱 (ESI-MS) 和核磁共振波谱 (NMR) 等方法进行化学结构分析。结果 从牡蛎肉中提取得到了3种牡蛎粗多糖,单糖组成都只含有葡萄糖 (Glc)。对粗多糖进一步分离,得到了5种多糖纯化组分。对水溶性多糖纯化组分 MC-11 的结构进行了分析,表明其是以 →4)-α-D-Glc-(1→ 为主链,含有 →3,4)- β-D-Glc-(1→和 →2,4)- β-D-Glc-(1→ 分支的 D-吡喃型葡聚糖,相对分子质量为1299 kDa。结论 从太平洋牡蛎肉中分离纯化得到了5种多糖组分,并采用多种方法确定了1种纯化组分 MC-11 的结构,为牡蛎多糖结构与活性关系的深入研究提供了基础。
英文摘要:
      Objective To extract and purify polysaccharides from Crassostrea gigas, and investigate their physical/chemical properties and structural features. Methods Crassostrea gigas polysaccarides were obtained from the acetone powder of Crassostrea gigas by water extraction at room temperature, 60℃ and by dilute alkali extraction sequentially. The contents of total sugar and protein were determined by colorimetric assay, and the monosaccharide compositions were analyzed by high performance liquid chromatography. The polysaccarides were purified by DEAE Sepharose FF anion-exchange and Sepharcryl S-400 gel-permeation chromatography, respectively. The structure of polysaccharides was characterized by FT-IR, methylation analysis, GC/MS, ESI-MS and NMR. Results Three crude polysaccharides were isolated from the acetone powder of Crassostrea gigas, and they are composed of only Glc. Five fractions were obtained after further isolated by column chromatography.The structure of MC-11 was characterized to be →4)-α-D-Glc-(1→, with little →3,4)- β-D-Glc-(1→ and →2,4)-β-D-Glc-(1→ branched units. Conclusion Five polysaccharides were separated from Crassostrea gigas and the structure of MC-11 was determined. These results will provide basis for further research on relationships of structure and activity of Crassostrea gigas polysaccharides.
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