| .蜈蚣藻多糖的体外抗血管生成作用[J].中国海洋药物,2010,29(1):. |
| 蜈蚣藻多糖的体外抗血管生成作用 |
| Anti-angiogenic effects of Grateloupia filicina polysaccharide in vitro |
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| DOI： |
| 中文关键词: 蜈蚣藻 人脐静脉内皮细胞 血管生成 |
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| 中文摘要: |
| 目的探讨蜈蚣藻多糖(GFP)的体外抗血管生成作用。方法应用MTT比色法测定GFP对人脐静脉内皮细胞(HUVEC)存活率的影响;用Millicell小室测定GFP对HUVEC迁移能力的影响;用Matrigel测定GFP对HUVEC管腔形成的影响。结果GFP在5、10、20、50、100mg.L-1浓度下处理HUVEC48h,细胞存活率没有显著性变化;在50、100mg.L-1时能显著性地抑制HUVEC的迁移并呈剂量依赖关系;在100mg.L-1时能显著性地抑制HUVEC的管腔形成。结论GFP能够抑制HUVEC的迁移和管腔的形成。 |
| English Summary: |
| Objective The present study was designed to observe the anti-angiogenic properties of Grateloupia filicina polysaccharide (GFP).Methods The survival rate of human umbilical vein endothelial cells (HUVEC) was assessed using MTT method after treating with GFP.The effect of GFP on HUVEC migration ability was observed using Millicell.The effect of GFP on HUVEC tube formation was assessed using Matrigel.Results The HUVEC cell viability did not change significantly after being treated with GFP for 48h at doses of... |
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