| .螺旋藻多糖对紫外线诱发的人胚肺二倍体细胞DNA损伤修复的影响[J].中国海洋药物,2001,20(2):27-31. |
| 螺旋藻多糖对紫外线诱发的人胚肺二倍体细胞DNA损伤修复的影响 |
| The influence of polysaaccharide from Spirulina platensis on human embryo lung diploid fibroblastic DNA damage and repair after UV irradiation |
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| DOI： |
| 中文关键词: 螺旋藻多糖 单细胞凝胶电泳 核酸内切酶 DNA损伤 DNA修复合成 二倍体细胞 |
| English Keywords:Polysaccharide from spirulina platensis,single cell gel electrophoresis,endonu- cluase,DNA damage,DNA repair synthesis |
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| 中文摘要: |
| 目的：观察螺旋藻多糖（polysaaccharide from Spirulina platensis,PSP)对紫外线诱发的人胚肺二倍体细胞DNA损伤修复的影响。方法：使用单细胞凝胶电泳技术（single cell gel clectrophoresis assay SCGE)，检测紫外线（UV）照射30min及照射后30,60,90min各组细胞DNA的损伤情况，以DNA迁移距离（migrate length of DNA,MLD)作为损伤指标。结果PSP能预防UV诱发的DNA损伤，并能促进UV诱发损伤后的DNA修复合成。结论：PSP有增强核酸内切酶和连接酶活性的作用，并且这种作用呈剂量依赖性。 |
| English Summary: |
| Objective To observe the influence of polysaccharide from spirulina platensis(PSP) on human embryo lung diploid fibroblastic DNA damage and repair after UV irradiation. Methods After UV irradiation for 30min, the single cell gel electrophoresis(SCGE) was used to investigate the level of DNA damage, the level of DNA damage and repair at 30, 60, 90min were also investigated by SCGE respectively. Results The level of DNA damage induced by UV irradiation was prevented by PSP, and it also promote DNA repair capacity. Conclusion PSP can increase the activity of DNA endonuclease and DNA ligase in dose-de- pendrntly. |
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