| .海洋放线菌细胞毒抗肿瘤活性物质的初筛[J].中国海洋药物,1999,18(2):52-55,27. |
| 海洋放线菌细胞毒抗肿瘤活性物质的初筛 |
| SCREENING OF CYTOTOXIC ANTITUMOR AGENTS FROM MA- RINE ACTINOMYCETES BY MTT ASSAY |
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| DOI： |
| 中文关键词: 海洋放线菌 抗肿瘤活性物质 体外筛选 |
| English Keywords:marine actinomycetes: antitumor agents,MTT assay,cytotoxicity,in vitro screening |
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| 中文摘要: |
| 本文以改进的ＭＴＴ法为初筛模型，筛选海洋放线菌产生的细胞毒抗肿瘤活性物质，对４３４株海洋放线菌发酵液的筛选结果发现，约１６％的海洋放线菌发酵液对Ｐ３８８和ＫＢ癌细胞的ＩＤ８０等于或大于３２０，其中最高可达２０４８０。这表明海洋放线菌存在着活性高的细胞毒抗肿瘤活性物质，是潜在的抗癌药源。 |
| English Summary: |
| The modified MTT assay was used as in vitro model for screening potential cy- totoxic antitumor agents produced by marine actinomycetes. Tile cytotoxicity of fermentation broth from 134 strains of marine actinomycetes on P_(388) and KB cancer cells was evaluated and the result showed that about 16% of fermentation broths had ID_(50) at or above 320,of which the highest ID_(50) is 20480,indicating that marine actinomycetes,which produced antitumor agents with strong cytotoxicity,are the potential source of antitumor drugs. |
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