.高产壳聚糖酶菌株的筛选及其降解壳聚糖反应初探[J].中国海洋药物,1999,18(2):. |
高产壳聚糖酶菌株的筛选及其降解壳聚糖反应初探 |
FILTRATION OF BEAUVERIA BASSIANA WITH HIGH-YIELD CHITOSANASE AND ITS ACTIVITY OF HYDROLYZING CHI- TOSAN |
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中文关键词: 球孢白僵菌,诱变,壳聚糖酶,壳寡糖 |
English Keywords:Beauverza bassiana,mutagenize,chitosanase,chitosan-oligosacchrides |
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中文摘要: |
通过两轮有效的紫外线诱变,获得了2株具有高产壳聚糖酶的突变株.具有较好的稳定性。其发酵液的粗酶活性达 0.2 U/ml,比原始保存突变菌株酶活性提高了 8倍。并得到一定量壳寡糖。 |
English Summary: |
Two high chitosanase producing mutant srains mutagenized by UV were obtained. The experimental results indicated that the highest-yield mutant's chitosanase activity in the fermentation liquid was 0. 2U/ml and 8 times higher than the original strain. Some certain kinds of chitosan oligosacchrides were obtained. |
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