| .海洋嗜盐微生物的研究开发：Ⅰ.嗜盐细菌产生的类胡萝卜素[J].中国海洋药物,1996,15(2):1-4. |
| 海洋嗜盐微生物的研究开发：Ⅰ.嗜盐细菌产生的类胡萝卜素 |
| RESEARCH ON MARINE HALOPHILIC MICROORGANISMS I Marine halophilic bacteria-produced carotenoids |
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| DOI： |
| 中文关键词: 嗜盐细菌 类胡萝卜素 菌红素 提取 鉴定 |
| English Keywords:halobacterium,carotenoids,bacterioruberin |
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| 中文摘要: |
| 分离纯化到几十株海洋嗜盐细菌，经筛选发现其中Ｆ－１１菌株，属于嗜盐杆菌属，能产生类胡萝卜素，在特的条件下发酵时，所产生的类胡萝卜素经提取和纯化，得朱红色的结晶，通过现代波谱技术的鉴定，确认是类胡萝卜素中的菌红素。除Ｆ－１１菌株外，还有一些待鉴定的菌株也能产生胡萝卜素或类胡萝卜素物质。 |
| English Summary: |
| Strain F-11 and several other strains of Halobacteria were isolated from saltern soils in China. Strain F-11 is Gram negative rod and obligate aerobe. Optimum salt concentration for strain F-11 growth is 16%-30% NaCl and no growth below 12% NaCl. And strain F-11 can produced carotenoids pigments. Therefore, strain F-11 is considered as one member of extreme halobacteria.
Natural pigment B1 is mainly produced by strain F-ll. Compound B1 is obtained as a red crystal and soluble in organic solvents, but no soluble in water. Compound Bl exhibits a molecular ion by EI-MS at m/z 740. 6, corresponding to the formula C50H76O4.
The UV and IR spectra of Bl is identical to that of bacterioruberin, which is one member of C-50 carotenoids. These data coupled with 'H NMR and MS spectral data allowed us to consider that compound Bl is identical with bacterioruberin. |
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