| .扇贝糖胺聚糖的琼脂糖凝胶电脉分析[J].中国海洋药物,1996,15(2):. |
| 扇贝糖胺聚糖的琼脂糖凝胶电脉分析 |
| IDENTIFICATION OF GLYCOSAMINOGLYCAN FROM THE BAY SCALLOP ARGOPECTEN IRRADIAUS BY AGAROSE GEL ELEC-TROPHORESIS |
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| 中文关键词: 扇贝,糖胺聚糖,琼脂糖凝胶电泳,分析 |
| English Keywords:Bay scallop (Argopecten irradiaus ),glycosaminoglycan,agarose gel elec-trophoresis,analysis |
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| 摘要点击次数: 2254 |
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| 中文摘要: |
| 从海湾扇贝边中提取分离出扇贝糖胺聚糖,其红外光谱与肝素类似。将扇贝糖胺聚糖制品在0.06mol/L,pH8.6巴比妥缓冲系统中进行琼脂糖凝胶电泳分析,发现其电泳图谱显示单一区带;与糖胺聚糖标准品——肝素、透明质酸和6-硫酸软骨素对照,其电泳相对迁移率与肝素量为接近。 |
| English Summary: |
| Fraction F3-3-3 of glycosaminoglycan was isolated from the leftover bits of Bay scallop Argopecten irradiaus . Infrared spectra of F3-3-3 was similar to that of heparin. Agarose gel electrophoresis of this fraction was performed in the system of barbital buffer (0. 06 mol/L,pH 8. 6). It was found that F3-3-3 showed single band in the electrophoresis. Compared with the agarose gel electrophoresis of standard glycosaminoglycan, such as heparin, hyaluronic acid and chondroitin 6-sulfate, it indicated that the relative migration rate of the fraction was close to that of heparin. |
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