吴萍萍,迟茜文,黄薇,等.海洋本草软珊瑚共附生真菌Aspergillus versicolor CGF9-1-2化学成分研究[J].中国海洋药物,2024,43(6):22-28.
海洋本草软珊瑚共附生真菌Aspergillus versicolor CGF9-1-2化学成分研究
Research on the Secondary Metabolites from Soft Coral-associated Fungus Aspergillus versicolor CGF9-1-2
投稿时间：2023-11-02  修订日期：2023-12-29
DOI：10.13400/j.cnki.cjmd.2024.06.002
中文关键词:  海洋本草软珊瑚  Aspergillus versicolor  生物碱  酚酸  结构鉴定
English Keywords:Marine herb soft coral-associated Aspergillus versicolor  alkaloid  phenolic acids  identification
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作者单位E-mail
吴萍萍 广州中医药大学中药学院 20211120238@stu.gzucm.edu.cn 
迟茜文 广州中医药大学中药学院 cxwdmn@qq.com 
黄薇 广东省中医院大学城分院药剂科 1637604475@qq.com 
徐文博 广州中医药大学中药学院 2021061220@stu.gzucm.edu.cn 
刘苏婷 广州中医药大学中药学院 2478866195@qq.com 
胡金姗 广州中医药大学中药学院 20191102120@stu.gzucm.edu.cn 
樊浩 广州中医药大学中药学院 20212110040@stu.gzucm.edu.cn 
张翠仙* 广州中医药大学中药学院 zhangcuixian@gzucm.edu.cn 
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中文摘要:
      摘 要：目的 对一株软珊瑚来源共附生真菌Aspergillus versicolor CGF9-1-2的次生代谢产物进行研究。方法 采用硅胶柱层析、ODS柱层析、HPLC等色谱分离方法对其代谢产物进行分离纯化，运用HR-ESI-MS、NMR、ORD等技术及对分离得到的化合物进行结构鉴定，并对其进行TDP1活性测试。结果 从Aspergillus versicolor CGF9-1-2中分离得到10个化合物，分别鉴定为(2S, 2"R, 3R, 3"E, 4E, 8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2"-hydroxyl-3"-octadecenoyl)amino-9-methyl-4,8-octadecadiene(1)、(4E, 8E)-N-2"-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4, 8-sphingadienine (2)、desoxybrevianamide E (3)、cyclo-(D-Pro-D-Phe) (4)、cyclo-(L-Pro-D-Leu) (5)、indole-3-carboxy-acid (6)、β-Carboline (7)、(7S)-(+)-Hydroxysydonic acid (8)、ent-Aspergoterpenin C (9)和(E)-7-deoxy-7,8-didehydro-12-hydroxysydonic acid (10)。所有化合物对TDP1均无明显抑制活性。结论 化合物2为首次从Aspergillus属真菌分离得到，化合物1为首次从Aspergillus versicolor真菌分离得到。
English Summary:
      Abstract: Objective To study the chemical structures of secondary metabolites from soft coral derived fungus Aspergillus versicolor CGF9-1-2. Methods The secondary metabolites were separated and purified by silica gel column chromatography, ODS column chromatography, Sephadex LH-20 column chromatography, and HPLC. The structures of isolated compounds were subjected to high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR), and optical rotation dispersion (ORD) spectroscopy. They were also tested for their TDP1 activity. Results From the culture of Aspergillus versicolor CGF9-1-2, we successfully isolated and identified two sphingoglycolipid compounds(2S, 2"R, 3R, 3"E, 4E, 8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2"-hydroxyl-3"-octadecenoyl)amino-9-methyl-4,8-octadecadiene (1), (4E, 8E)-N-2"-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4, 8-sphingadienine (2), five alkaloids: desoxybrevianamide E (3), cyclo-(D-Pro-D-Phe) (4), cyclo-(L-Pro-D-Leu) (5), indole-3-carboxylic-acid (6), and β-Carboline (7). In addition, three simple phenolic acid compounds (7S)-(+)-Hydroxysydonic acid (8), ent-Aspergoterpenin C (9) and (E)-7-deoxy-7,8-didehydro-12-hydroxysydonic acid (10). None of these compounds showed significant TDP1 inhibitory activity. Conclusion 2 was firstly isolated from Aspergillus, and 1 was obtained from Aspergillus versicolor for the first time.
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