| 王周云,司玉玺,杨荣荣,等.可溶性透明质酸微针的制备及表征[J].中国海洋药物,2024,43(5):55-61. |
| 可溶性透明质酸微针的制备及表征 |
| Preparation and characterization of soluble hyaluronic acid microneedles |
| 投稿时间：2023-04-07 修订日期：2023-05-19 |
| DOI：10.13400/j.cnki.cjmd.2024.05.009 |
| 中文关键词: 透明质酸 微针 机械性能 |
| English Keywords:Hyaluronic acid Microneedles Mechanical properties |
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| 中文摘要: |
| 目的 以透明质酸和聚乙烯吡咯烷酮为基质材料,制备机械强度优良的微针材料(HA/PVP-MN),并考察微针的透皮能力和生物相容性。方法 以分子量为40 kDa的透明质酸为基质材料,加入合适比例的聚乙烯吡咯烷酮,在两步离心、室温干燥条件下制备微针。通过形态学观察、皮肤溶解性实验、皮肤穿透实验和细胞活力实验,考察透明质酸微针针尖穿透皮肤的能力和生物相容性。结果 扫描电镜观察到微针阵列完整,针尖高度约为750 μm,机械性能测试表明在透明质酸和聚乙烯吡咯烷酮质量比为2 : 1时,针尖强度最优且具有良好的溶解性能,微针能有效穿透皮肤角质层,插入皮肤深度约为220 μm；将透明质酸和聚乙烯吡咯烷酮与人永生化角质细胞共培养24 h后,细胞仍可保持较高活力,表明微针基质材料具备良好的生物相容性。结论 制备了一种新型基于透明质酸的微针递药系统,发现该微针能有效穿透皮肤角质层并具备良好的生物相容性。 |
| English Summary: |
| Objective To prepare HA/PVP-MN with excellent mechanical strength and investigate its transdermal ability and biocompatibility using hyaluronic acid and polyvinylpyrrolidone as matrix materials. Methods Hyaluronic acid with molecular weight of 40 kDa was used as matrix material, and polyvinylpyrrolidone was added in the appropriate proportion. The microneedle array was prepared under the condition of two-step centrifugation and drying at room temperature. The skin penetration ability and biocompatibility of HA microneedles were evaluated by morphological observation, skin solubility test, skin penetration test and cell viability test. Results SEM showed that the morphology of the microneedle array was intact, and the tip height was about 750 μm. Mechanical properties test showed that the microneedle array had the best tip strength and good dissolution performance when the mass ratio of hyaluronate and polyvinylpyrrolidone was 2 : 1. After co-cultured with hyaluronic acid and polyvinylpyrrolidone for 24 hours, human immortalized keratinocytes still maintained a high vitality, indicating that the matrix material had good biocompatibility. Conclusion A novel microneedle drug delivery system based on HA is successfully prepared. The microneedles can effectively penetrate the cuticle and have good biocompatibility. |
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