| 宫雪,李志源,时全,等.斑马鱼Sgo1在眼睛发育中的作用及调控机制研究[J].中国海洋药物,2024,43(5):62-68. |
| 斑马鱼Sgo1在眼睛发育中的作用及调控机制研究 |
| Study on the role and regulation mechanism of zebrafish Sgo1 in eye development |
| 投稿时间：2023-03-21 修订日期：2023-05-04 |
| DOI：10.13400/j.cnki.cjmd.2024.05.001 |
| 中文关键词: Sgo1 眼睛 细胞凋亡 斑马鱼 |
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| 中文摘要: |
| 目的 SGO1突变与人类隐性遗传病——CAID综合征（Chronic Atrial Intestinal Dysrhythmia syndrome）相关。为详细探究SGO1/Sgo1的体内生理作用及分子机制，运用模式动物斑马鱼进行分析。方法 通过CRISPR/Cas9基因编辑技术构建sgo1敲除突变体；显微成像观察Sgo1缺失对斑马鱼眼睛生长发育的影响；qRT-PCR（实时荧光定量PCR）及冰冻切片免疫组化实验检测斑马鱼Sgo1缺失对视网膜光感受器发育的影响；吖啶橙染色显示斑马鱼眼睛细胞凋亡水平；qRT-PCR检测p53信号通路靶基因表达水平。结果 斑马鱼Sgo1功能缺失影响眼睛视觉转导，同时能够激活p53信号通路，导致眼部细胞凋亡增加从而影响眼睛正常发育。 |
| English Summary: |
| Objective SGO1 mutation is associated with CAID syndrome (Chronic Atrial Intestinal Dysrhythmia syndrome). In order to explore the physiological role and molecular mechanism of SGO1/Sgo1 in vivo, this study used zebrafish as a model organism for analysis. Methods Using CRISPR/Cas9 gene editing technology to construct sgo1 knockout mutant; observe the growth and development of zebrafish eyes by microscopic imaging; conducting qRT-PCR and frozen section immunohistochemistry to detect the effects of zebrafish Sgo1 deletion on the development of retinal photoreceptors; using acridine orange staining showed the apoptosis level of zebrafish eyes and conducting qRT-PCR to detect the expression levels of target genes in p53 signaling pathway. Conclusion The loss of zebrafish Sgo1 function affects the visual transduction of the eyes and activate the p53 signaling pathway resulting in increased apoptosis, which affecting the normal development of the eyes. |
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