李超,王远红,杨爽,等.海参药材质量标准提升的研究[J].中国海洋药物,2022,41(5):65-72.
海参药材质量标准提升的研究
Research on Improving the Quality Standard of Sea Cucumber
投稿时间：2021-05-10  修订日期：2021-08-30
DOI：
中文关键词:  海参药材  质量标准  薄层色谱法  多糖  特征图谱。
English Keywords:sea cucumber medicinal materials  quality standard  TLC  polysaccharide  characteristic chromatogram.
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作者单位E-mail
李超 中国海洋大学 海洋药物教育部重点实验室，山东省糖科学与糖工程重点实验室 2234259438@qq.com 
王远红 中国海洋大学 海洋药物教育部重点实验室，山东省糖科学与糖工程重点实验室 yhwang@ouc.edu.cn 
杨爽* 中国海洋大学 海洋药物教育部重点实验室，山东省糖科学与糖工程重点实验室；青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室 yangshuang@ouc.edu.cn 
吕志华 中国海洋大学 海洋药物教育部重点实验室，山东省糖科学与糖工程重点实验室；青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室 lvzhihua@ouc.edu.cn 
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中文摘要:
      目的 制定山东省地方习用药材海参的质量标准。方法 参照《中国药典》（2020版）相关方法对海参药材进行性状和薄层色谱（TLC）鉴别研究，测定了水分、酸不溶性灰分及浸出物的含量；通过硫酸苯酚法以葡萄糖为对照品测定海参多糖的含量；采用高效液相色谱法（HPLC）对海参药材的特征图谱进行研究。结果 不同批次海参药材的性状特征较一致；TLC斑点清晰，重复性好；海参药材中水分的质量分数为8.5% ~ 9.5%，酸不溶性灰分的质量分数为0.1% ~ 0.2%，浸出物的质量分数范围为33.6% ~ 51.7%；以葡萄糖计海参多糖的质量分数范围为0.52% ~ 0.85%；供试品色谱图中呈现的5个特征峰与对照药材参照物色谱图中的5个特征峰的保留时间相对应。结论 本研究建立的定性及定量方法操作简便、准确、稳定。可用于海参药材的质量控制。
English Summary:
      Objective To establish the quality standards of the local medicinal sea cucumber in Shandong Province. Methods According to the relevant methods of the Chinese Pharmacopoeia (2020 edition), the properties and thin-layer identification chromatography of sea cucumber medicinal materials were studied. The content of water, acid-insoluble ash and extracts were determined. The content of sea cucumber polysaccharides was determined by ultraviolet-visible spectrophotometry. The characteristic chromatogram of sea cucumber medicinal materials was studied using the HPLC method. Results The traits of different batches of sea cucumber medicinal materials were relatively consistent. TLC spots were clear and reproducible. The water content in sea cucumber medicinal materials was 8.5% ~ 9.5%, the acid-insoluble ash content was 0.1% ~ 0.2%, and the extract content was 33.6% ~ 51.7%. Calculated by glucose, sea cucumber polysaccharide content was 0.52% ~ 0.85%. The retention time of the five characteristic peaks in the chromatogram of the test samples corresponds to that of the reference medicinal material. Conclusion The method established in this study is simple, accurate and stable, and can be used for the quality control of sea cucumber medicinal materials.
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