| 于雅琳,胡志飞,汤震.真菌Penicillium sp. DT-F27次级代谢产物及其活性研究[J].中国海洋药物,2021,40(2):21-27. |
| 真菌Penicillium sp. DT-F27次级代谢产物及其活性研究 |
| Study on the secondary metabolites and their antitumor bioactivities of a marine-derived fungus Penicillium sp. DT-F27. |
| 投稿时间：2020-09-04 修订日期：2020-10-26 |
| DOI： |
| 中文关键词: Penicillium sp. 次级代谢产物 抗肿瘤 |
| English Keywords:Penicillium sp. Secondary metabolites Antitumor |
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| 中文摘要: |
| 摘要：目的 一株海洋来源真菌Penicillium sp. DT-F27次级代谢产物及其抗肿瘤活性的研究。方法 采用硅胶柱色谱、反相高效液相制备色谱等方法，对该真菌发酵产物的乙酸乙酯提取物进行分离纯化；采用波普解析方法对化合物的结构进行鉴定；采用MTT法测定化合物对人前列腺癌PC-3细胞的抗肿瘤作用。结果 从真菌Penicillium sp. DT-F27的发酵物中分离得到15个化合物，分别为麦角甾醇（1），dehydrocyclopeptine（2），3-O-methylviridicatin（3），verrucofortine（4），cyclopenin（5），cyclo(dehydroala-L-Leu)（6），cyclopenol（7），4-hydroxy-2-methoxy acetanilide（8），trans-(3RS,4RS)-3,4-dihydro-3,4,8-trihydroxynaphthalen-1(2H)-one（9），cyclo(D-Pro-D-Val)（10），brevianamide F（11），cyclo(L-Pro-L-Val)（12），anacine（13），cyclo(L-Orn-L-Tyr-L-Orn-L-Ala)（14），cyclo(L-Pro-L-Tyr)（15）。结论 从真菌Penicillium sp. DT-F27的次级代谢产物中分离15化合物，其中麦角甾醇（1）和3-O-methylviridicatin（3）具有较强的抗肿瘤活性，抑制率分别为45.6%和34.8%。 |
| English Summary: |
| Abstract: Objective To study the secondary metabolites and their cytotoxic bioactivities of a marine-derived fungus Penicillium sp. DT-F27. Methods silica gel column chromatography and reversed-phase high performance liquid chromatography were used to isolate and purify the compounds from the ethyl acetate extract from the fungus fermentation products. Structures of compounds were analyzed by spectroscopic analysis. MTT assay was used to determine the antitumor effect of the compounds on human prostate cancer PC-3 cells. Results fifteen compounds were isolated from Penicillium sp. DT-F27 and structures were determined as ergosterol (1), dehydrocyclopeptine (2), 3-Omethylviridicatin (3), verrucofortine (4), cyclopenin (5), cyclo(dehydroala-L-Leu) (6), cyclopenol (7), 4-hydroxy-2-methoxy acetanilide (8), trans-(3RS,4RS)-3,4-dihydro-3,4,8-trihydroxynaphthalen-1(2H)-one (9), cyclo(D-Pro-D-Val) (10), brevianamide F (11), cyclo(L-Pro-L-Val) (12), anacine (13), cyclo(L-Orn-L-Tyr-L-Orn-L-Ala) (14), cyclo(L-Pro-L-Tyr) (15). Conclusion fifteen compounds were isolated from the fermentation products of Penicillium sp. DT-F27, of which ergosterol (1) and 3-O-methylviridicatin (3) had the strong antitumor activity of 45.6% and 34.8% respectively. |
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