| 刘俊丽,代明琴,杨昭,等.虾青素/天然DNA/壳聚糖纳米粒对紫外诱导的小鼠皮肤光老化的改善作用[J].中国海洋药物,2019,38(4):32-38. |
| 虾青素/天然DNA/壳聚糖纳米粒对紫外诱导的小鼠皮肤光老化的改善作用 |
| Effect of Astaxanthin/Natural DNA/Chitosan Nanoparticles on UV-induced Photoaging of Mouse Skin |
| 投稿时间：2019-03-25 修订日期：2019-04-30 |
| DOI： |
| 中文关键词: 虾青素 纳米粒 紫外 光老化 皮肤 |
| English Keywords:astaxanthin nanoparticles ultraviolet photoaging skin |
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| 中文摘要: |
| 目的 探究虾青素/天然DNA/壳聚糖纳米粒（Ast/DC）对紫外诱导的小鼠皮肤光老化的改善作用。方法 分子自组装结合溶剂蒸发法制备Ast/DC。紫外照射脱毛小鼠30 d模拟皮肤光老化，研究Ast/DC对小鼠皮肤表观形态、组织纤维结构以及皮肤中超氧化物歧化酶（SOD）等抗氧化因子表达水平等的影响；并用Franz扩散池模拟透皮吸收过程研究纳米载体对虾青素的经皮递送效果。结果 将平均粒径为266 nm、Zeta电位为32.7 mV的Ast/DC（虾青素有效剂量为1.5 μg?cm-2?d-1）涂抹于紫外照射小鼠的背部，可维持其皮肤正常形态和结构，在角质层厚度、胶原纤维和弹性纤维结构等方面与未经紫外照射小鼠相比无明显差异；并且皮肤组织中的谷胱甘肽（GSH）、SOD和过氧化氢酶（CAT）表达水平较光老化小鼠分别显著提高31.4%、25.1%和41.7%（P＜0.05）。当虾青素有效涂抹剂量低至0.33 μg?cm-2?d-1时，Ast/DC仍能显著提高皮肤组织中的抗氧化因子表达水平，且优于游离虾青素；相较于光老化模型小鼠，低剂量Ast/DC组小鼠和虾青素油（Ast/oil）组小鼠中GSH含量分别提高26.4%和15.1%。同时，Ast/DC的24 h累积透皮吸收量比游离虾青素提高20.7%。结论Ast/DC能有效保护小鼠皮肤，抵御紫外照射引起的皮肤光老化。 |
| English Summary: |
| Objective To investigate the effect of astaxanthin/natural DNA/chitosan nanoparticles (Ast/DC) on ultraviolet-induced photoaging of mouse skin. Methods Ast/DC were prepared by self-assembly and solvent evaporation method. Ultraviolet light was used to irradiate the depilatory mice skin for 30 days to simulate skin photoaging process. The effects of Ast/DC on protecting skin from ultraviolet-induced photoaging were studied by evaluating the apparent morphology of the skin, the tissue and fiber structure, as well as the content of the antioxidant factors in skin tissue, etc. The Franz diffusion cell was used to simulate the transdermal absorption process of Ast/DC. Results The Ast/DC nanoparticles were successfully prepared with an average particle size of 266 nm, and the Zeta potential of the nanoparticles was 32.7 mV. By applying Ast/DC (1.5 μg?cm-2?d-1 of equivalent astaxanthin) to the ultraviolet-irradiated mice skin, the skin appearance and structure could be maintained. There was no obvious difference on the thickness of epidermis, the structure of collagen fibers and elastic fibers between Ast/DC-treated mice and non-ultraviolet irradiated mice. Besides, the content of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) in the skin tissues were significantly improved by 31.4%, 25.1% and 41.7% (P＜0.05), compared with the skin of photoaging mice. When given the same astaxanthin dose of 0.33 μg?cm-2?d-1, the increasing rate of GSH in the Ast/DC group (26.4%) was higher than that of astaxanthin oil group (15.1%). Similar results could be found in the terms of SOD and CAT. Ex vivo transdermal absorption study showed that the cumulative permeability of astaxanthin in Ast/DC in 24 hours was 20.7% higher than that in astaxanthin oil. Conclusion Ast/DC can efficiently protect mice skin from ultraviolet-induced photoaging. |
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