徐明远,肖菲,李花月,等.色氨酸分解代谢途径阻断对深海链霉菌ZH66次级代谢及生长的影响[J].中国海洋药物,2018,37(6):8-14.
色氨酸分解代谢途径阻断对深海链霉菌ZH66次级代谢及生长的影响
The effect of tryptophan catabolic pathway blocking on secondary metabolism and growth of deepsea-derived Streptomyces somaliensis SCSIO ZH66
投稿时间：2018-05-29  修订日期：2018-06-23
DOI：
中文关键词:  深海链霉菌,色氨酸分解代谢,色氨酸双加氧酶,次级代谢
English Keywords:deepsea-derived Streptomyces, tryptophan catabolism, Tryptophan-2, 3-dioxgenase, secondary metabolism
Fund Project:
作者单位E-mail
徐明远 中国海洋大学海洋药物教育部重点实验室 xumingyuan1993@qq.com 
肖菲 中国海洋大学海洋药物教育部重点实验室  
李花月 中国海洋大学海洋药物教育部重点实验室  
鞠建华 中国科学院南海海洋研究所 中国科学院热带海洋生物资源与生态重点研究室 广东省海洋药物重点实验室  
李文利* 中国海洋大学海洋药物教育部重点实验室 liwenli@ouc.edu.cn 
摘要点击次数: 3826
全文下载次数: 943
中文摘要:
      【目的】以深海链霉菌Streptomyces somaliensis SCSIO ZH66为研究对象,通过阻断其色氨酸分解代谢途径,探究代谢产物与生长的变化。【方法】通过Blastp分析寻找S. somaliensis SCSIO ZH66基因组上编码色氨酸双加氧酶基因tdo(ORF0630和ORF6017),在前期阻断ORF0630的基础上采用PCR-targeting的策略阻断ORF6017,通过HPLC检测发酵产物的变化,并观察用不同培养基培养时突变株与野生株生长的差别。【结果】与野生株相比,突变株ZH66Δtdo不产生antimycins,但无其他次级代谢产物的变化。与此同时,ZH66Δtdo在MS平板上开始产生气生菌丝与孢子的时间均提前了12 h,在ISP-2液体培养基中生长对数期开始时间同样提前12 h。【结论】链霉菌S. somaliensis SCSIO ZH66中色氨酸分解代谢途径的阻断未促进其他可能以色氨酸为前体次级代谢产物的合成,而是促进了菌株的生长,为其他链霉菌中色氨酸分解代谢调控提供了借鉴。
English Summary:
      [Objective] To investigate the change of secondary metabolism as well as growth after blocking the tryptophan catabolic pathway in deepsea-derived Streptomyces somaliensis SCSIO ZH66. [Methods] Tryptophan-2, 3-dioxgenase encoding genes tdo (ORF0630 and ORF6017) were identified from the genome of S. somaliensis SCSIO ZH66 by Blastp search. ORF6017 was knocked out using PCR-targeting strategy based on previously constructed ORF0630 gene inactivation mutant, resulting in ZH66Δtdo. The fermentation products of ZH66Δtdo and wild type strains were analyzed by HPLC, and their growth was observed after cultivation on plates and in liquid media. [Results] HPLC analysis suggested production of antimycins was abolished in ZH66Δtdo , but no other difference was observed in comparison with the wild type strain. However, formation of the aerial hyphae on MS medium as well as start of the exponential phase in ISP-2 liquid medium of ZH66Δtdo were both 12 h earlier than that of the wild type strain. [Conclusion] The disruption of tryptophan catabolic pathway in S. somaliensis SCSIO ZH66 didn’t cause production of tryptophan-derived secondary metabolites, but accelerated the growth of the mutant strain. These results provided a reference for the researches of tryptophan catabolism regulation in other Streptomyces strains.
查看全文  查看/发表评论  下载PDF阅读器
关闭