| .具有抗肿瘤活性的海洋链霉菌WBF7发酵条件的初步研究[J].中国海洋药物,2007,26(2):34-37. |
| 具有抗肿瘤活性的海洋链霉菌WBF7发酵条件的初步研究 |
| Preliminary research on fermentation conditions of marine Streptomyces WBF7 with antitumor activity |
| 修订日期：2006-09-04 |
| DOI： |
| 中文关键词: 链霉茵属 抗肿瘤活性 发酵条件 |
| English Keywords:WBF7 |
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| 中文摘要: |
| 目的对海洋链霉菌WBF7的发酵条件进行优化．提高抗肿瘤活性物质的产量。方法采用单因素实验、正交实验，应用MTT法，检测发酵液活性的变化．对其发酵条件进行优化。结果抗肿瘤活性代谢产物产生的最优发酵培养基确定为：蔗糖4％，马铃薯20％，酵母粉0．4％和氯化钙0．18％，陈海水．pH7．2。最佳发酵时间为7d。结论以最优发酵条件发酵WBF7，其发酵液的活性明显提高。 |
| English Summary: |
| Objective To optimize fermentation conditions of marine streptornyces WBF7 with antitumor activity.Methods Fermentation conditions were optimized using single-factor ex- periment and the orthogonal matrix method on the base of activity(ID_(50))of broth by MTT assay.Results Optimum fermentation conditions of antitumor metabolizes production:su- crose 4%,potato 20%,yeast extract 0.4%,CaCl_2 0.18%,sea water,adjusted to pH 7.2, and optimum fermentation time 7d.Conclusion The antitumour activity of the fermentation liquid of marine Streptornyces WBF7 was significantly increased under the given optimum conditions. |
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