| .一种无血清培养动脉环血管生成改良方法的建立[J].中国海洋药物,2001,20(1):52-54. |
| 一种无血清培养动脉环血管生成改良方法的建立 |
| The establishment of a modified method to evaluate angiogenesis of rat rings in serum free medium |
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| DOI： |
| 中文关键词: 主动脉环 血管生成 大鼠 无血清培养 |
| English Keywords:Angiogenesis,Modified method,Aorta rings,Wistar rats,MCDB131 medium |
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| 中文摘要: |
| 改良大鼠主动脉环培养生成血管方法。采用Wistar大鼠主动脉环，纤维蛋白原包埋于48孔板中，MCDB131无血清培养液培养。结果表明主动脉环生长良好，第4天血管芽开始从动脉壁长出，第6～13天进入生长期，第13天达108条左右，第13天后新生血管数不再增多。 |
| English Summary: |
| The establishment of a modified method to evaluate angiogenesis of rat rings in serum-free medium was investigated in this paper. The aorta rings of Wistar rats were embedded in fibrinogen gel in 48 well plates, and cultured in an optimized serum free medium MCDB131. Results indicated that new capillaries spouted on 4th day, and entered into growth phase from 6th day, and peaked on 13th day and remained unchanged thereafter. |
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