| 韦梦焱,刘小琳,樊懿乐,等.多孔壳聚糖/海藻酸钠/β-磷酸三钙支架的制备及初步安全性评价[J].中国海洋药物,2026,(5):-. |
| 多孔壳聚糖/海藻酸钠/β-磷酸三钙支架的制备及初步安全性评价 |
| Preparation and preliminary safety evaluation of porous chitosan/sodium alginate/β-TCP scaffold |
| 投稿时间：2025-04-25 修订日期：2025-06-16 |
| DOI： |
| 中文关键词: 多孔支架 海藻酸钠 壳聚糖 组织工程 抗菌活性 |
| English Keywords:porous scaffold sodium alginate chitosan tissue engineering antibacterial activity |
| Fund Project: |
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| 中文摘要: |
| 目的 制备基于海藻酸钠(AL)、壳聚糖(CS)和β-磷酸三钙(β-TCP)的新型复合多孔支架,评价其结构特性、生物相容性及抗菌性能。方法 采用冷冻干燥法制备AL/CS/β-TCP支架,通过SEM观察形貌并测定孔径与孔隙率,并通过抑菌圈实验和细菌生长曲线检测抗菌活性。采用CCK-8法测定支架对L929和NIH-3T3细胞活力的影响,评价其体外生物相容性。结果 制备的支架呈现互联多孔结构,优化组平均孔径129.6 ± 12 μm,孔隙率84.0 ± 3%。体外实验显示,该支架对细胞无明显毒性(活力> 80%),并具备抗菌活性。结论 AL/CS/β-TCP支架具有良好的物理化学性能、生物相容性和抗菌作用,在抗菌型组织工程支架中具有潜在应用价值。 |
| English Summary: |
| Objective To fabricate novel porous scaffolds composed of sodium alginate (AL), chitosan (CS), and β-tricalcium phosphate (β-TCP), and evaluate their structural characteristics, biocompatibility, and antibacterial properties. Methods AL/CS/β-TCP scaffolds were prepared using the freeze-drying method. Scanning electron microscopy (SEM) was employed to observe the morphology and determine pore size and porosity. Antibacterial activity was assessed using the zone of inhibition test and bacterial growth curve analysis. In vitro biocompatibility was evaluated by measuring the viability of L929 and NIH-3T3 cells cultured with the scaffolds using the CCK-8 assay. Results The fabricated scaffolds exhibited an interconnected porous structure. Optimized scaffolds demonstrated a mean pore size of 129.6 ± 12 μm and a porosity of 84.0 ± 3%. In vitro experiments indicated that the scaffolds possessed no significant cytotoxicity (cell viability > 80%) and exhibited antibacterial activity. Conclusion The AL/CS/β-TCP scaffolds possess favorable physicochemical properties, good biocompatibility, and antibacterial effects, suggesting their potential application value as antibacterial tissue engineering scaffolds. |
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