林丽远,唐燕飞,杨胜涛,等.等鞭金藻多肽IEC对脂多糖诱导血管内皮炎症的保护作用研究[J].中国海洋药物,2022,(6):-.
等鞭金藻多肽IEC对脂多糖诱导血管内皮炎症的保护作用研究
The Protective Effect of Polypeptide IEC from Isochrysis zhangjiang on LPS induced vascular endonal inflammation
投稿时间:2021-06-27  修订日期:2021-10-05
DOI:
中文关键词:  等鞭金藻  多肽  HUVEC  抗炎  脂多糖
English Keywords:Isochrysis  polypeptide  HUVECs  anti-inflammatory  LPS
Fund Project:
作者单位邮编
林丽远 广东海洋大学化学与环境学院 524088
唐燕飞 广东海洋大学化学与环境学院 
杨胜涛 广东海洋大学食品科技学院 
千忠吉 广东海洋大学化学与环境学院 524088
摘要点击次数: 184
全文下载次数: 0
中文摘要:
      目的 探究氨基酸序列为Ile-Ile-Ala-Val-Glu-Ala-Gly-Cys的等鞭金藻多肽IEC对LPS诱导人脐静脉内皮细胞(HUVEC)炎症反应的保护作用。方法 用噻唑蓝(MTT)法确定细胞活力,DCFH-DA探针法和DAF-FM DA探针法分别测定活性氧(ROS)和NO的表达情况,蛋白免疫印迹法检测细胞间Toll样受体(TLR4)、一氧化氮合酶(NOS-2)和环氧合酶(COX-2)蛋白的表达水平,酶联免疫吸附法测定白细胞介素-6 (IL-6)和肿瘤坏死因子(TNF-α)的释放量以及分子对接分析IEC与COX-2、TLR-4蛋白的相互作用。结果 MTT法证明IEC对HUVEC细胞无明显毒性作用(P>0.05);与对照组相比,随着实验组多肽浓度增加,可以明显降低ROS和NO的释放(P<0.05),炎症相关细胞因子TLR-4、COX-2、NOS-2和IL-6、TNF-α的表达也明显逐渐减少(P<0.000 1);分子对接结果表明,IEC与COX-2、TLR4能形成稳定的化学键,说明存在相互作用的可能性。结论 IEC具有良好的抑制ROS和NO生成效果,并可明显抑制TLR-4/NOS-2/COX-2信号通路的激活以及抑制炎症因子的表达,是潜在的炎症抑制剂。
English Summary:
      Objective To explore the protective effect of the Isochrysis polypeptide IEC with amino acid sequence Ile-Ile-Ala-Val-Glu-Ala-Gly-Cys on LPS-induced inflammatory response in human umbilical vein endothelial cells (HUVEC). Methods MTT method was used to determine cell viability, DCFH-DA probe method and DAF-FM DA probe method to measure the expression of reactive oxygen species (ROS) and nitric oxide (NO), respectively, and Western blotting was used to detect intercellular Toll-like receptors (TLR4), nitric oxide synthase (NOS-2) and Cyclooxygenase (COX-2) protein expression level, enzyme-linked immunosorbent assay to determine the release of interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) and molecular docking analysis IEC and COX- 2. The interaction of TLR-4 protein. Results The MTT method proved that IEC had no obvious toxic effect on HUVEC cells (P>0.05); compared with the control group, as the peptide concentration of the experimental group increased, the release of ROS and NO could be significantly reduced (P<0.05), inflammation-related cytokine TLR- 4. The expressions of COX-2, NOS-2, IL-6, and TNF-α are also significantly reduced (P<0.000 1); the molecular docking results show that IEC, COX-2 and TLR4 can form stable chemical bonds, indicating that there is a mutual Possibility of function. Conclusion IEC has a good effect of inhibiting ROS and NO generation, and can significantly inhibit the activation of TLR-4/NOS-2/COX-2 signaling pathway and inhibit the expression of inflammatory factors. It is a potential inhibitor of inflammation.
  查看/发表评论  下载PDF阅读器
相关附件:   作者承诺  修改说明
关闭